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. 1991 Sep 11;19(17):4775. doi: 10.1093/nar/19.17.4775

Use of polymerase chain reaction for rapid detection of gene insertions in whole yeast cells.

G M Sathe 1, S O'Brien 1, M M McLaughlin 1, F Watson 1, G P Livi 1
PMCID: PMC328729  PMID: 1653951

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Güssow D., Clackson T. Direct clone characterization from plaques and colonies by the polymerase chain reaction. Nucleic Acids Res. 1989 May 25;17(10):4000–4000. doi: 10.1093/nar/17.10.4000. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Koltin Y., Faucette L., Bergsma D. J., Levy M. A., Cafferkey R., Koser P. L., Johnson R. K., Livi G. P. Rapamycin sensitivity in Saccharomyces cerevisiae is mediated by a peptidyl-prolyl cis-trans isomerase related to human FK506-binding protein. Mol Cell Biol. 1991 Mar;11(3):1718–1723. doi: 10.1128/mcb.11.3.1718. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Mullis K. B., Faloona F. A. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods Enzymol. 1987;155:335–350. doi: 10.1016/0076-6879(87)55023-6. [DOI] [PubMed] [Google Scholar]
  4. Rose M., Grisafi P., Botstein D. Structure and function of the yeast URA3 gene: expression in Escherichia coli. Gene. 1984 Jul-Aug;29(1-2):113–124. doi: 10.1016/0378-1119(84)90172-0. [DOI] [PubMed] [Google Scholar]
  5. Rothstein R. J. One-step gene disruption in yeast. Methods Enzymol. 1983;101:202–211. doi: 10.1016/0076-6879(83)01015-0. [DOI] [PubMed] [Google Scholar]

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