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. 1991 Sep 25;19(18):4937–4941. doi: 10.1093/nar/19.18.4937

Determination of the DNA-interacting region of the archaebacterial chromosomal protein MC1. Photocrosslinks with 5-bromouracil-substituted DNA.

M Katouzian-Safadi 1, B Laine 1, F Chartier 1, J Y Cremet 1, D Belaiche 1, P Sautiere 1, M Charlier 1
PMCID: PMC328793  PMID: 1923761

Abstract

Protein MC1 is the major chromosomal protein in methanosarcinaceae. Using photochemical crosslinking on 5-bromouracil-substituted DNA, we identified the region of the protein that interacts with it. This region is located in the C-terminal part of the polypeptide chain, and the crosslinked amino-acids are in the region 74-86. Tryptophan 74 is one of the amino-acids crosslinked to DNA.

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Selected References

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