Skip to main content
. Author manuscript; available in PMC: 2012 Aug 23.
Published in final edited form as: Oncogene. 2011 Jul 18;31(8):1034–1044. doi: 10.1038/onc.2011.296

Figure 2. MiR-20a/miR-17-5p-dependent regulation of ZBTB4.

Figure 2

Effects of miR-20a and miR-17-5p and their antagomirs on interactions with wild-type ZBTB4-3’-UTR (A and B) and mutant (C) constructs. MCF-7 or MDA-MB-231 cells were transfected with miR-20a and miR-17-5p mimics or antagomirs (100 nM) and wild-type or mutant constructs containing ZBTB4 3’-UTR inserts linked to the luciferase gene, and after 48 hr, luciferase activity was determined as described in the Materials and Methods. (D) As-miR-20a induces ZBTB4 expression. MCF-7 or MDA-MB-231 cells were transfected with as-miR-20a (150 nM) and after 48 hr and expression of ZBTB4 protein and mRNA was determined as described in the Materials and Methods. Results are means ± SE (triplicate) and significant (p<0.05) induction (*) or inhibition (**) is indicated.