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. Author manuscript; available in PMC: 2012 Aug 23.
Published in final edited form as: Oncogene. 2011 Jul 18;31(8):1034–1044. doi: 10.1038/onc.2011.296

Figure 5. Decreased Sp1, Sp3, Sp4 and Sp-regulated protein expression by ZBTB4 overexpression or endogenous miR-20a knockdown and EMSA results.

Figure 5

Effects of ZBTB4 expression (A) and as-miR-20a (B) on Sp1, Sp3 and Sp4 protein expression. MCF-7 or MDA-MB-231 cells were transfected with ZBTB4 (0, 1 or 2 µg) or as-miR-20a (0, 100 or 150 nM), and whole cell lysates were analyzed for Sp proteins by western blots as described in the Materials and Methods. Effects of ZBTB4 (C) and as-miR-20a (D) on Sp-regulated genes and PARP cleavage. Cells were transfected with either ZBTB4 or as-miR-20a, and whole cell lysates were analyzed by western blots as described in the Materials and Methods.