Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Phenotype | Source of reference |
|---|---|---|
| V. Vulnificus strains | ||
| CMCP6 | Wild type clinical isolate | Kim and Rhee (2003) |
| AA-16 | ΔvenB, ΔtonB1, ΔtonB2, ΔtonB3 | Alice et al. (2008) |
| AA-14 | ΔvenB | Alice et al. (2008) |
| VSSD5 | ΔvenB, ΔhvtR | This study |
| VSSD25 | ΔvenB, ΔhupA | This study |
| VSSD40 | ΔvenB, ΔhvtR, ΔhupA | This study |
| VSSD91 | VSSD40 harboring pMMB208-hvtR | This study |
| VSSD74 | ΔhvtR, ΔhupA | This study |
| VSSD61 | ΔvenB, ΔtonB1 | This study |
| VSSD62 | ΔvenB, ΔtonB2 | This study |
| VSSD53 | ΔvenB, ΔttpC2 | This study |
| VSSD57 | ΔvenB, ΔtonB1, ΔtonB2 | This study |
| VSSD64 | ΔvenB, ΔtonB1, ΔttpC2 | This study |
| VSSD92 | VSSD74 harboring pMMB208 | This study |
| VSSD93 | VSSD74 harboring pMMB208-hvtR | This study |
| E. Coli strains | ||
| TOP10 | F− mcrA Δ(mrr-hsdRMS-mcrBC) ϕ 80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL (Strr) endA1 nupG | Invitrogen |
| S17-1λpir | Λpir lysogen; thi pro hsdR hsdM+ recA RP4-2 Tc:Mu-Km:Tn7(Tpr Smr) | Simon et al. (1983) |
| Plasmids | ||
| PCR II Blunt | Blunt end cloning vector: Kmr | Invitrogen |
| pPCR2.1 | TA cloning vector; Ampr, Kmr | Invitrogen |
| pDM4 | Suicide vector with oriR6K; CmrsacB | Milton et al. (1996) |
| pRK2013 | Helper plasmid; Kmr | Figurski and Helinski (1979) |
| pMMB208 | Broad-host-range expression vector; Cmr Ptac | Morales et al. (1991) |
| phupA | pDM4-ΔhupA | Alice et al. (2008) |