Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1991 Oct 25;19(20):5561–5567. doi: 10.1093/nar/19.20.5561

Compositional bimodality of the nuclear genome of tobacco.

G Matassi 1, R Melis 1, G Macaya 1, G Bernardi 1
PMCID: PMC328957  PMID: 1658735

Abstract

We have studied the compositional distribution of six genes (or small multigene families) and of one family of transposable elements, Tnt1, in DNA fractions from tobacco (Nicotiana tabacum) separated according to base composition. We have shown that gene distribution is bimodal and that such bimodality is due to the different base composition of the two parental genomes of tobacco (N.sylvestris and N.tomentosiformis) and to the different parental origin of the genes tested. These results indicate a physical separation and an absence of extensive recombination of the parental genomes, which have been together in the tobacco nucleus for a small span of their evolutionary life, and a conservation of their compositional patterns, including gene localization.

Full text

PDF
5561

Images in this article

5562Image
on p.5562
5562Image
on p.5562
5563Image
on p.5563
5563Image
on p.5563
5563Image
on p.5563
5564Image
on p.5564
5564Image
on p.5564

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aïssani B., D'Onofrio G., Mouchiroud D., Gardiner K., Gautier C., Bernardi G. The compositional properties of human genes. J Mol Evol. 1991 Jun;32(6):493–503. doi: 10.1007/BF02102651. [DOI] [PubMed] [Google Scholar]
  2. Bennett M. D., Smith J. B. Nuclear dna amounts in angiosperms. Philos Trans R Soc Lond B Biol Sci. 1976 May 27;274(933):227–274. doi: 10.1098/rstb.1976.0044. [DOI] [PubMed] [Google Scholar]
  3. Bernardi G., Bernardi G. Codon usage and genome composition. J Mol Evol. 1985;22(4):363–365. doi: 10.1007/BF02115693. [DOI] [PubMed] [Google Scholar]
  4. Bernardi G., Bernardi G. Compositional transitions in the nuclear genomes of cold-blooded vertebrates. J Mol Evol. 1990 Oct;31(4):282–293. doi: 10.1007/BF02101123. [DOI] [PubMed] [Google Scholar]
  5. Bernardi G., Mouchiroud D., Gautier C., Bernardi G. Compositional patterns in vertebrate genomes: conservation and change in evolution. J Mol Evol. 1988 Dec;28(1-2):7–18. doi: 10.1007/BF02143493. [DOI] [PubMed] [Google Scholar]
  6. Bernardi G., Olofsson B., Filipski J., Zerial M., Salinas J., Cuny G., Meunier-Rotival M., Rodier F. The mosaic genome of warm-blooded vertebrates. Science. 1985 May 24;228(4702):953–958. doi: 10.1126/science.4001930. [DOI] [PubMed] [Google Scholar]
  7. Bernardi G. The isochore organization of the human genome. Annu Rev Genet. 1989;23:637–661. doi: 10.1146/annurev.ge.23.120189.003225. [DOI] [PubMed] [Google Scholar]
  8. Cameron D R. Inheritance in Nicotiana Tabacum. Xxiv. Intraspecific Differences in Chromosome Structure. Genetics. 1952 May;37(3):288–296. doi: 10.1093/genetics/37.3.288. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Cortadas J., Macaya G., Bernardi G. An analysis of the bovine genome by density gradient centrifugation: fractionation in Cs2SO4/3,6-bis(acetatomercurimethyl)dioxane density gradient. Eur J Biochem. 1977 Jun 1;76(1):13–19. doi: 10.1111/j.1432-1033.1977.tb11565.x. [DOI] [PubMed] [Google Scholar]
  10. Gleba Y. Y., Parokonny A., Kotov V., Negrutiu I., Momot V. Spatial separation of parental genomes in hybrids of somatic plant cells. Proc Natl Acad Sci U S A. 1987 Jun;84(11):3709–3713. doi: 10.1073/pnas.84.11.3709. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Grandbastien M. A., Spielmann A., Caboche M. Tnt1, a mobile retroviral-like transposable element of tobacco isolated by plant cell genetics. Nature. 1989 Jan 26;337(6205):376–380. doi: 10.1038/337376a0. [DOI] [PubMed] [Google Scholar]
  12. Gray J. C., Kung S. D., Wildman S. G. Origin of Nicotiana tabacum L. detected by polypeptide composition of Fraction I protein. Nature. 1974 Nov 15;252(5480):226–227. doi: 10.1038/252226a0. [DOI] [PubMed] [Google Scholar]
  13. Heslop-Harrison J. S., Bennett M. D. Nuclear architecture in plants. Trends Genet. 1990 Dec;6(12):401–405. doi: 10.1016/0168-9525(90)90300-u. [DOI] [PubMed] [Google Scholar]
  14. Jamet E., Durr A., Fleck J. Absence of some truncated genes in the amphidiploid Nicotiana tabacum. Gene. 1987;59(2-3):213–221. doi: 10.1016/0378-1119(87)90329-5. [DOI] [PubMed] [Google Scholar]
  15. Kemp J. D., Sutton D. W. A chemical and physical method for determining the complete base composition of plant DNA. Biochim Biophys Acta. 1976 Mar 4;425(2):148–156. doi: 10.1016/0005-2787(76)90020-4. [DOI] [PubMed] [Google Scholar]
  16. Kirk J. T. Effect of methylation of cytosine residues on the buoyant density of DNA in caesium chloride solution. J Mol Biol. 1967 Aug 28;28(1):171–172. doi: 10.1016/s0022-2836(67)80087-1. [DOI] [PubMed] [Google Scholar]
  17. Lagrimini L. M., Burkhart W., Moyer M., Rothstein S. Molecular cloning of complementary DNA encoding the lignin-forming peroxidase from tobacco: Molecular analysis and tissue-specific expression. Proc Natl Acad Sci U S A. 1987 Nov;84(21):7542–7546. doi: 10.1073/pnas.84.21.7542. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Lee K. Y., Townsend J., Tepperman J., Black M., Chui C. F., Mazur B., Dunsmuir P., Bedbrook J. The molecular basis of sulfonylurea herbicide resistance in tobacco. EMBO J. 1988 May;7(5):1241–1248. doi: 10.1002/j.1460-2075.1988.tb02937.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Matassi G., Montero L. M., Salinas J., Bernardi G. The isochore organization and the compositional distribution of homologous coding sequences in the nuclear genome of plants. Nucleic Acids Res. 1989 Jul 11;17(13):5273–5290. doi: 10.1093/nar/17.13.5273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Mohnen D., Shinshi H., Felix G., Meins F. Hormonal regulation of beta1,3-glucanase messenger RNA levels in cultured tobacco tissues. EMBO J. 1985 Jul;4(7):1631–1635. doi: 10.1002/j.1460-2075.1985.tb03830.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Montero L. M., Salinas J., Matassi G., Bernardi G. Gene distribution and isochore organization in the nuclear genome of plants. Nucleic Acids Res. 1990 Apr 11;18(7):1859–1867. doi: 10.1093/nar/18.7.1859. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Mouchiroud D., D'Onofrio G., Aïssani B., Macaya G., Gautier C., Bernardi G. The distribution of genes in the human genome. Gene. 1991 Apr;100:181–187. doi: 10.1016/0378-1119(91)90364-h. [DOI] [PubMed] [Google Scholar]
  23. Salinas J., Matassi G., Montero L. M., Bernardi G. Compositional compartmentalization and compositional patterns in the nuclear genomes of plants. Nucleic Acids Res. 1988 May 25;16(10):4269–4285. doi: 10.1093/nar/16.10.4269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Shinshi H., Mohnen D., Meins F. Regulation of a plant pathogenesis-related enzyme: Inhibition of chitinase and chitinase mRNA accumulation in cultured tobacco tissues by auxin and cytokinin. Proc Natl Acad Sci U S A. 1987 Jan;84(1):89–93. doi: 10.1073/pnas.84.1.89. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Shinshi H., Neuhas J. M., Ryals J., Meins F., Jr Structure of a tobacco endochitinase gene: evidence that different chitinase genes can arise by transposition of sequences encoding a cysteine-rich domain. Plant Mol Biol. 1990 Mar;14(3):357–368. doi: 10.1007/BF00028772. [DOI] [PubMed] [Google Scholar]
  26. Shinshi H., Wenzler H., Neuhaus J. M., Felix G., Hofsteenge J., Meins F. Evidence for N- and C-terminal processing of a plant defense-related enzyme: Primary structure of tobacco prepro-beta-1,3-glucanase. Proc Natl Acad Sci U S A. 1988 Aug;85(15):5541–5545. doi: 10.1073/pnas.85.15.5541. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Sperisen C., Ryals J., Meins F. Comparison of cloned genes provides evidence for intergenomic exchange of DNA in the evolution of a tobacco glucan endo-1,3-beta-glucosidase gene family. Proc Natl Acad Sci U S A. 1991 Mar 1;88(5):1820–1824. doi: 10.1073/pnas.88.5.1820. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Vaucheret H., Vincentz M., Kronenberger J., Caboche M., Rouzé P. Molecular cloning and characterisation of the two homologous genes coding for nitrate reductase in tobacco. Mol Gen Genet. 1989 Mar;216(1):10–15. doi: 10.1007/BF00332224. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES