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. 1991 Oct 25;19(20):5603–5606. doi: 10.1093/nar/19.20.5603

Polymorphism in N-2-acetylaminofluorene induced DNA structure as revealed by DNase I footprinting.

X Veaute 1, R P Fuchs 1
PMCID: PMC328963  PMID: 1945836

Abstract

In this paper, we have constructed double stranded helices (60-mers) containing a single N-2-acetylaminofluorene (-AAF) adduct covalently bound to one of the three guanine residues of the Narl site (G1G2CG3CC). This sequence was identified as a strong frameshift mutation hot spot for many carcinogens that bind to the C8 position of guanine. Using DNase I as a probe for DNA conformation we show i) that the average size of the helix deformation extends over 3 to 5 base pairs in both directions from the adduct site, and ii) that there is a strong polymorphism in the adduct induced DNA conformation. The present study supports the idea that adducts induce specific sequence dependent local conformational changes in DNA that are differentially recognized and processed by the enzymatic machineries that lead to repair or mutagenesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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