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. 2012 Feb 29;7(2):e31898. doi: 10.1371/journal.pone.0031898

Genomic Diversity in Two Related Plant Species with and without Sex Chromosomes - Silene latifolia and S. vulgaris

Radim Cegan 1,2, Boris Vyskot 1, Eduard Kejnovsky 1, Zdenek Kubat 1, Hana Blavet 1, Jan Šafář 3, Jaroslav Doležel 3, Nicolas Blavet 4, Roman Hobza 1,*
Editor: Michael Freitag5
PMCID: PMC3290532  PMID: 22393373

Abstract

Background

Genome size evolution is a complex process influenced by polyploidization, satellite DNA accumulation, and expansion of retroelements. How this process could be affected by different reproductive strategies is still poorly understood.

Methodology/Principal Findings

We analyzed differences in the number and distribution of major repetitive DNA elements in two closely related species, Silene latifolia and S. vulgaris. Both species are diploid and possess the same chromosome number (2n = 24), but differ in their genome size and mode of reproduction. The dioecious S. latifolia (1C = 2.70 pg DNA) possesses sex chromosomes and its genome is 2.5× larger than that of the gynodioecious S. vulgaris (1C = 1.13 pg DNA), which does not possess sex chromosomes. We discovered that the genome of S. latifolia is larger mainly due to the expansion of Ogre retrotransposons. Surprisingly, the centromeric STAR-C and TR1 tandem repeats were found to be more abundant in S. vulgaris, the species with the smaller genome. We further examined the distribution of major repetitive sequences in related species in the Caryophyllaceae family. The results of FISH (fluorescence in situ hybridization) on mitotic chromosomes with the Retand element indicate that large rearrangements occurred during the evolution of the Caryophyllaceae family.

Conclusions/Significance

Our data demonstrate that the evolution of genome size in the genus Silene is accompanied by the expansion of different repetitive elements with specific patterns in the dioecious species possessing the sex chromosomes.

Introduction

Angiosperm species display large variability in genome size, ranging from 1C = 0.0648 pg in the carnivorous species Genlisea margarethae [1] to 1C = 152.23 pg in the monocot Paris japonica [2]. Even genomes of closely related species vary significantly in size and structure and this phenomenon was termed C-value paradox [3]. Such patterns are especially obvious in plants where genome dynamism is higher than in animals. During the past few decades the components contributing to differences in genome size were characterized. However, the reason for the variation remains unclear and the term C-value enigma was introduced to reflect this [4].

The major mechanisms of genome size increase are polyploidization [5] and the expansion of different repetitive elements [6], [7], [8]. In this work, we focus on the latter mechanism. Transposable elements (TEs) are widespread in all eukaryotes and may have a significant impact on genome size dynamics [9]. They have been classified in two separate classes based on their mode of transposition [10]. The first class contains elements transposing via an RNA intermediate. This class is further divided based on terminal sequences and includes long-terminal repeat (LTR) retrotransposons (Ty1/copia-like and Ty3/gypsy-like), transposons terminating at the 3′ end with a poly(A) tract (LINEs - long interspersed nuclear elements, SINEs - short interspersed nuclear elements), and other orders like DIRS (Dictyostelium intermediate repeat sequence) and PLE (Penelope-like elements) retrotransposons [10]. The proliferation of LTR-retrotransposons has been recognized as one of the key mechanisms accounting for genome expansion in plants [11]. The second class of TEs is transposed via a DNA intermediate. It has been grouped into several superfamilies such as hAT, CACTA, Mutator-like or Tc1/Mariner.

Another important mechanism of plant genome expansion is the proliferation of satellite DNA [12], [13]. In addition to many conserved repeated units including rDNA and telomeric and centromeric repeats, there is a large variation in other types of tandemly organized DNA that form plant genomes. Tandem repeats are arranged in microsatellites (2–5 bp units), minisatellites (up to 100 bp per unit), and larger satellites.

In this study, we investigate genome size evolution by comparing two closely related species from the Caryophyllaceae family, Silene latifolia and S. vulgaris. Both species belong to the genus Silene (subgenus Behenantha). S. latifolia Poiret (syn. Melandrium album Garcke, syn. Melandrium pratense Roehl.) is a strictly dioecious species with separate male and female individuals determined by the sex chromosomes. S. vulgaris is a gynodioecious species with female and hermaphroditic individuals. In this species the sex is controlled by interactions of nuclear (autosomal) factors and mitochondrial genes in the process called cytoplasmic male sterility [14].

The structure and evolution of the entire S. latifolia genome has been studied more extensively, mainly in the context of sex chromosome evolution [15], [16]. Many tandem repeats in S. latifolia are localized in the vicinity of telomeres [17], [18], [19] and/or specifically linked to the sex chromosomes [20], [21]. A comprehensive study of microsatellite distribution in S. latifolia [22] relied on fluorescence in situ hybridization (FISH) and showed that specific sequence repeats are overrepresented on the Y chromosome. It has also been demonstrated that chloroplast DNA had been transferred and accumulated on S. latifolia sex chromosomes during their evolution [23]. The authors identified chloroplast sequences in Y chromosome specific genomic library. Moreover, FISH results with BAC clone containing part of chloroplast genome revealed strong signal intensity on the Y chromosome. Matsunaga et al. [24], Obara et al. [25], and Kejnovsky et al. [26] studied the structure and evolution of specific retrotransposons in S. latifolia. Pritham et al. [27] isolated the first transcriptionally active DNA transposon linked to the Y chromosome. A systematic study of repetitive DNA in S. latifolia showed that Copia retroelements are probably the most abundant DNA element on the Y chromosome [28]. The first active MITE (miniature inverted-repeat transposable element) elements in S. latifolia were described by Bergero et al. [29].

These studies reflect the increasing data about the structure of S. latifolia genome mainly with a special focus on sex chromosome evolution; however, there has not been a comparison among closely related species to distinguish which types of elements played a role in genome size evolution generally, and which elements played a role in sex chromosome evolution specifically. For S. vulgaris there is almost no data about the structure and evolution of major repetitive elements in its genome. There exists only fragmented information about the differences between S. latifolia and S. vulgaris genomes, which comes from studies focused on the characterization of some gene regions [30], [31].

Here we address the following questions: What makes the S. latifolia genome larger in comparison with S. vulgaris? Is the smaller genome of S. vulgaris a priori with a lower copy number of individual repetitive elements? Do all repetitive elements amplify in a bigger genome? Is there a space for the large duplication events that might have formed S. latifolia genome? Is S. latifolia genome larger just based on expansion of repetitive DNA compared to S. vulgaris? Which DNA elements play a specific role in formation of the sex chromosomes?

Results

Differences in the abundance of individual repetitive elements in S. latifolia and S. vulgaris genome

We constructed a short-insert DNA library of S. vulgaris. The library contained 7,720 clones with an average insert size of 603 bp representing an equivalent of about 0.42% of S. vulgaris genome (1C = 1.13 pg DNA = 1102.50 Mbp [32]). In order to isolate clones containing repetitive elements, TEs and tandem repeats, we amplified the conservative domains of gypsy and copia-like reverse transcriptases, LINE endonuclease, Au SINE, CACTA, Mariner and Mutator transposase, STAR-C, TR1 and X.43.1 tandem repeats and used them as probes for a hybridization-based library screening. We applied a complex genomic DNA probe in parallel to identify other repetitive elements. After screening the library, all positively hybridizing clones were sequenced and the presence of specific repeats was confirmed. The number of clones containing each of the individual repeats is summarized in Table S1. In order to make our data comparable with those obtained by Cermak et al. [28] for S. latifolia, we calculated the relative number of clones containing specific elements and the percentage of the genome formed by this element (Table 1).

Table 1. The total number of clones from the S. vulgaris short insert library containing respective elements and the proportion of total screened clones in comparison with the S. latifolia [28].

Type of element
Silene vulgaris Silene latifolia *
number of sequences per genome % of genome number of sequences per genome % of genome Ratio %SV/%SL
Retroelements 29540 22.9% 72500 36.4% 0.63
LTR 29064 22.8% 69687 36.1% 0.63
Gypsy 13112 12.7% 55937 32.5% 0.39
Athila 714 0.5% 10000 2.7% 0.18
Ogre 0 0 25000 22.7% 0
Peabody 1904 1.1% 11250 2.8% 0.39
Retand 11190 11.2% 9687 4% 2.8
Copia 15952 10.1% 13750 3.6% 2.81
Non - LTR 476 0.10% 2813 0.3% 0.33
SINE 0 0 0 0 0
LINE 476 0.10% 2813 0.3% 0.33
DNA transposons 1428 0.6% 938 0.2% 3
Tandem repeats 42141 0.3% 42814 0.46% 0.65
Tandem repeat STAR 28095 0.1% 9063 0.02% 5
Tandem repeat X.43.1 4523 0.12% 31563 0.37% 0.32
TR1 9523 0.06% 2188 0.01% 6
All repetitive elements 73109 23.4% 116252 36.8% 0.64
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Ratios % SV/% SL below 1.0 indicate elements that are more expanded in S. latifolia genome, and ratios % SV/% SL higher than 1.0 show elements that are more expanded in S. vulgaris.

As in the S. latifolia genome, retroelements represented the most repetitive fraction of S. vulgaris genome. However, the genome fraction comprised by retroelements was 1.6× higher in S. latifolia than in S. vulgaris, indicating that retroelements have played a dominant role in the expansion of the S. latifolia genome. The contribution of individual retroelements differed significantly. The most extreme values were observed for the Ogre retroelement group which is present in 25 thousands of copies in the S. latifolia genome (∼23% of its genome). Based on shotgun genomic library screening this element is absent in S. vulgaris genome. On the other hand, the Retand retroelement represented ∼11% of S. vulgaris genome compared to only 4% in S. latifolia. The Athila and Peabody retroelements were also more abundant in S. latifolia. The Copia type retrotransposons represented ∼10% of S. vulgaris genome compared to ∼4% in S. latifolia. Non-LTR (SINE, LINE) retrotransposons appear to have played only a minor role in genome size divergence between the species. DNA transposons are about 2× more common in S. vulgaris compared to S. latifolia, but as with non-LTR retrotransposons they represent only a minor fraction of the genome in both species. Surprisingly, centromeric tandem repeats STAR-C and TR1 are almost 5× more abundant in S. vulgaris than in S. latifolia. The opposite is true of the subtelomeric tandem repeat X.43.1, which is 3× more abundant in the S. latifolia genome. Although the percentage of these elements in the genome seems to be rather low in both species, this percentage does not take into account tandem (i.e. the number of repeat units) in individual sequenced clones (one clone was calculated as one hit) since such data is not yet available for S. latifolia. Taken together, the majority of the TEs were more abundant in the S. latifolia genome. On the other hand, some TEs like Retand or STAR-C centromeric tandem repeat formed a larger proportion of the S. vulgaris genome. Overall, the repetitive elements we identified in the S. vulgaris and S. latifolia genomes represented ∼23% and ∼36% of entire genome, respectively.

We used Southern blot hybridization to estimate the abundance of Ogre, Retand, and Copia retroelements in both the S. latifolia and S. vulgaris genomes. We used the reverse transcriptase domain for Ogre and Copia retroelements, and the pol domain for Retand as a probes. This hybridization data clearly showed that the Ogre retroelement was missing in the S. vulgaris genome (Figure S1). The Retand and Copia retroelements are similarly abundant in both genomes. Except for the Ogre retroelement, the hybridization patterns of all the studied elements are similar in both species. Comparisons of hybridization signals between genomic DNA restricted by the methylation sensitive HpaII enzyme and its isoschizomer MspI showed that these three analyzed elements are partially methylated in both genomes.

Chromosomal distribution and evolutionary divergence of repetitive DNA

To show patterns of individual repetitive elements on chromosomes of S. latifolia, S. vulgaris and some other closely related species, we employed FISH with the most abundant repeats (Figure 1). The probes used for FISH spanned the conservative parts of individual repetitive elements identified by genomic library screening. Based on abundance we selected Ogre and Retand elements (gypsy retrotransposons), Copia retroelements, and tandem repeats STAR-C and TR1 for further experiments. The 25S rDNA and X.43.1 repeats were used as internal controls. We selected five species from the Caryophyllaceae family (genus Silene: S. latifolia, S. dioica, S. vulgaris; genus Lychnis: L. chalcedonica, genus Dianthus: D. caryophyllus) for FISH experiments. Our aim in this set of experiments was to compare the distribution and organization of these elements in order to make inferences about both genome and sex chromosome evolution.

Figure 1. Chromosomal distribution of the reverse transcriptases of Ogre, Retand and Copia retroelements in five species from the Caryophyllaceae family as revealed by FISH.

Figure 1

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Mitotic metaphase chromosomes of S. latifolia (A, F, K), S. dioica (B, G, L), S. chalcedonica (C, H, M), S. vulgaris (D, I, N) and Dianthus caryophyllus (E, J, O) were counterstained with DAPI (blue). The probes for each element (Ogre – reverse transcriptase (A–E), Retand – reverse transcriptase (F–J) and Copia – reverse transcriptase (K–O)) were labeled with Cy3-conjugated nucleotides (red). The probe for X.43.1. subtelomeric repeat (A, B, F, G, K, L) was labeled with Spectrum Green (green). The X and Y chromosomes are indicated, bar indicates 10 µm.

The Ogre retroelement, the most abundant repetitive sequence in S. latifolia, showed, in agreement with the previously published data [28], [33], a random distribution over all the chromosomes, but almost no signal on the Y chromosome. We observed a similar pattern in a closely related dioecious plant S. dioica. Interestingly, we found no evidence of the Ogre retroelement in L. chalcedonica, S. vulgaris and D. caryophyllus. The Retand retroelement, which was linked to the subtelomeric region in S. latifolia [26], was localized to the terminal chromosomal regions of all the chromosomes in S. latifolia, S. dioica as well as in S. vulgaris in agreement with a previous report [26]. In D. caryophyllus only several major signals were observed. Surprisingly, completely different pattern of distribution was found in L. chalcedonica; almost all the chromosomes possessed strong centromeric signals with no hybridization signals in the terminal parts of the chromosomes. Copia retrotransposons showed a relatively evenly spread distribution in all chromosomes (with the exception of a missing signal in the subtelomeric regions) in all studied species.

The tandem repeat STAR-C was localized at the centromeres in all studied species (Figure S2). Only D. caryophyllus had some chromosomes without any signal. In order to show differences in abundance of STAR-C in S. latifolia and S. vulgaris (∼5× more abundant in the S. vulgaris genome compared to S. latifolia (Table 1)), we took images of metaphase chromosomes after FISH hybridization with STAR-C probe with different exposure times (Figure S3). This “quantitative” FISH of STAR-C confirmed that this repeat was more abundant in S. vulgaris. The TR1 tandem repeat showed subtelomeric organization corresponding to rDNA regions in S. latifolia, S. dioica, S. vulgaris, and L. chalcedonica. Co-localization of TR1 with rDNA loci was previously demonstrated by Cermak et al. in S. latifolia [28]. The only exception was D. caryophyllus, where we did not find any TR1 hybridization. The X.43.1 tandem repeat was subtelomerically organized in the S. latifolia, S. dioica and S. vulgaris genomes. In L. chalcedonica X.43.1 was localized only at several dominant subtelomeric and minor centromeric loci. In D. caryophyllus X.43.1 was completely missing (Figure S2).

Comparative analysis of complete Retand retrotransposons from S. latifolia and S. vulgaris

We compared the sequence of Retand-2 [26] of S. latifolia with the Retand element from BAC clone 62M2 from S. vulgaris containing Sv4 gene previously characterized in this species [31], [34]. We measured coverage of both elements with 454 sequencing reads [35] obtained from S. latifolia male and female genomes (Figure 2). Retand-2 was homogenously represented in both male and female genomic 454 reads with higher abundance of LTRs suggesting the presence of solo LTRs in S. latifolia genome (upper part of Figure 2). The Retand element of S. vulgaris origin exhibited the highest degree of identity with S. latifolia 454 reads in the gag-pol region (lower part of Figure 2). It was in accordance with alignment of both Retand elements showing their highest similarity in the central region (middle part of Figure 2).

Figure 2. Comparison of S. latifolia (Retand-2) and S. vulgaris (BAC62M2) Retand retrotransposons.

Figure 2

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Retand elements are represented on the X axis with indicated length in bp. The plots represent genomic copy numbers of individual insert regions calculated from numbers of similarity hits to the 454 read databases [35] of both male (SLM-all, blue) and female (SLF-all, red) S. latifolia individuals. The central panel displays a comparison of similarity of individual retroelements. Specific domains of retroelements are indicated.

Analysis of microsatellite sequences in corresponding genomic loci in S. latifolia and S. vulgaris

To analyze the extent and pattern of distribution of different microsatellite sequences, we counted all di-, tri- and tetra- microsatellites (Table S2) in two gene regions linked to the sex chromosomes in S. latifolia (Sl4X/Y, SlAP3X/Y) and the corresponding autosomal region in S. vulgaris. BAC clones for all these loci were previously identified and characterized by Marais et al. [30] and Cegan et al. [31]. Separately we summarized data on dinucleotides CA, GA, GC and trinucleotides CAG, CAG, GAA, TAA, which were shown to be accumulated on Y chromosome of S. latifolia [22]. However, we did not find a specific accumulation of any microsatellite type in the Y linked sequences compared to the X and autosomal loci. Longer stretches of microsatellites (>18 bp) were almost completely missing in the analyzed sequences (Table S3). In the SlAP3Y gene linked genomic locus, the largest dinucleotide array (AC) was composed of 50 bp, trinucleotide of 48 bp (GAA), and tetranucleotide of 42 bp (ATAC). In the Sl4Y gene linked genomic locus, the largest dinucleotide array (TA) was composed of 54 bp, trinucleotide of 96 bp (TTA), and tetranucleotide of 16 bp (ACAA). Taken together, the abundance of microsatellites was similar in BAC clones from homologous regions in S. latifolia and S. vulgaris, without any accumulation on the sex chromosomes. Some of the microsatellites that are known to accumulate on the Y chromosome in S. latifolia [22] formed the longest arrays in the studied BAC clones (AC, TA, GAA).

Discussion

What makes the S. latifolia genome larger than the S. vulgaris genome?

It is known that retrotransposons play a dominant role in genome size evolution in both angiosperm and in gymnosperm species [36]. To get a profile of the most abundant DNA sequences in S. vulgaris, we employed two different strategies. First, we amplified the conservative parts of different retrotransposons and other repeats found and characterized in closely related species and used amplicons as the probes for hybridization with the random genomic library. To identify other abundant elements specific to the S. vulgaris genome we screened the genomic DNA in parallel. We avoid the next generation sequencing (NGS) methods to make our data comparable to Cermak et al. [28] who used identical methodical approach in S. latifolia. Moreover, a recent paper comparing 454 based estimation of repetitive DNA proportion in S. latifolia with data by Cermak et al. [28] reveals similar results and conclusions. Even though NGS data could provide deeper insight into medium repetitive sequences, occurrence of tandem repeats with hairpin like structures in Silene genomes [20] could be underestimated due to sequencing problems. Generally, it is known that sequencing of satellite repeats leads to underestimation of their abundance [37]. Furthermore, NGS methods could bias quantification of individual repetitive elements by occurrence of multiple identical reads [38] and lower efficiency in GC-rich templates sequencing [39].

By comparing the S. vulgaris and S. latifolia genomes we showed that the gypsy retroelement Ogre was the most divergent repetitive sequence, with almost no occurrence in the smaller genome of S. vulgaris and with a total count of about 25,000 in the S. latifolia genome (Table 1). Surprisingly, this element was not observed in the non-recombining part of the Y chromosome, suggesting that this element was not a major player in the evolution of large sex chromosomes in the dioecious S. latifolia.

The Retand retrotransposon was the most abundant repetitive DNA in S. vulgaris comprising a higher proportion of the S. vulgaris genome than in S. latifolia. Using BAC clones we have identified a full length Retand retroelement from the S. vulgaris genome and compared this sequence to the known Retand sequence from S. latifolia [26]. Our data clearly show that the gag and pol parts share about 80% similarity between the species, while the LTRs and ORF3, and ORF4 are much more divergent (Figure 2). Our findings are supported by the fact that, for comparison, we used a complex set of 454 data from S. latifolia [35]. High divergence of LTR sequences even in such closely related species could present a useful tool for studies of recent evolution of sex chromosomes via colonization by retrotrasposons. Especially dating and character of divergence between sex chromosomes can be precised by combination of gene based data with LTR evolutionary dynamics. The Retand element was found in the vicinity of the SlAP3 gene, which could also suggest that gene rich regions co-localize with subtelomeres where Retand is a dominant element in both studied species. These data are in agreement with observation by Siroky et al. [40] showing, that euchromatin markers like early replication and H4 hyperacetylation can be detected at all subterminal chromosome regions.

Copia retrotransposons are the third most abundant elements in S. latifolia and the second more abundant in the S. vulgaris genome. The widespread presence of this element and its conserved pattern of distribution in these two species corresponds to observed data in other plant species [41].

The data presented here show that specific groups of repetitive elements have differentially proliferated in two closely related Silene species. Our data further suggest that transposable element expansion alone is not sufficient to explain genome size evolution and genome size differences between S. latifolia and S. vulgaris. Our data show that all of the repetitive elements observed in this study together cover ∼22% of S. vulgaris genome and ∼37% of entire genome in S. latifolia (Table 1). Which mechanisms of genome formation could explain this discrepancy? It has been shown that variation in intron length differs significantly between X and Y linked genes and their counterparts in autosomal loci in S. vulgaris [30]. This process along with other suggested mechanisms such as expansion/contraction of tandem repeats, illegitimate recombination [42], and different numbers of genes between species [43] are all candidates for explaining differences in genome formation and should be studied in further detail in these species.

What if the genome of S. vulgaris was reduced compared to S. latifolia? It has been shown that genomic DNA loss by unequal homologous recombination and illegitimate recombination of retrotransposons occurred in rice [44]. Evidence of a similar mechanism has been found in Arabidopsis species [43]. In both studies it was shown that the reduction partially affected all retroelements. This differs from our observation in Silene species that some retroelements display similar numbers between species (Copia, Retand) and some retroelements differ significantly (Ogre). These data suggest that genome size reduction has not been a key mechanism of genome size evolution in S. vulgaris and in S. latifolia since these species diverged from common ancestor.

What is the chromosomal distribution of individual elements in related species?

Since the haploid chromosome number (n) is 12 in almost all Silene species, there is no evidence of large-scale reorganization or even polyploidization during the recent evolution of the genus. We expected that due to the stability of genomes in the Silene genus our data would only reveal patterns of expansion of individual repeats in various species. For our experiments, we selected related species with sex chromosomes (S. dioica) as well as two other species from Caryophyllaceae family, one with a small genome (Dianthus caryophyllus) and the other with a large genome (Lychnis chalcedonica) [32], [45]. Surprisingly, we found very distinct patterns of distribution of the Retand retroelement in these species. Although this element has strict subtelomeric organization in S. latifolia, S. dioica and S. vulgaris, its centromeric position in Lychnis chalcedonica reveals that large chromosomal rearrangements followed by fusion-fission events might have occurred during the evolution of Caryophyllaceae family (Figure 1). To confirm centromeric localization of Retand in Lychnis chalcedonica we performed bicolor FISH using both Retand and STAR-C probes (Figure S4). On the other hand, using telomeric probe for FISH experiments doesn't reveal occurrence of telomeric sequences in internal or centromeric parts of chromosomes (data not shown). Since there is limited amount of genetic and genomic data for Lychnis chalcedonica, further detailed study should be carried out to show cause of large chromosome evolution in this species.

Ogre retroelements are present only in dioecious species from the section Melandrium, in S. latifolia and in S. dioica. Surprisingly, this element was not found in the Y chromosomes. These data suggest that the expansion of this element is a very recent evolutionary event specific for the section Melandrium. It also suggests that the mode of retrotransposition could be connected with recombination machinery, which is not present in the non-recombining part of the Y chromosome.

The chromosomal distribution of Copia retroelements is conserved in all the species examined in this study. The ability of FISH probes to hybridize even on the chromosomes of more distinct species suggests low sequence divergence of the element during evolution. It further seems that Copia retroelements have been very stable in their numbers per genome during the long term evolution of the Silene species. The ratio of the total counts of Copia elements in S. vulgaris to S. latifolia is 2.7 (Table 1). This is almost exactly the same as the ratio between the genome sizes of these two species, suggesting that Copia retroelements keeps their copy numbers in the genome at least 7 Mya, which is the age of the oldest stratum in the sex chromosomes of S. latifolia [34]. The high sequence similarity of Copia retroelements could be a reason that these sequences are recognized en bloc by RNAi machinery and effectively silenced. On the other hand, the Copia retrotransposon is the most accumulated retroelement on the Y chromosome of S. latifolia [28]. This fact could suggest that there exist other mechanism(s) removing Copia elements apart from the Y chromosome in S. latifolia genome.

The tandem repeat STAR-C displayed a conserved centromeric pattern in all of the species from the Caryophyllaceae family. Along with TR1, these two tandem repeats are the most biased elements in S. vulgaris compared to S. latifolia in terms of total numbers. Since it is known that tandem repeats are amplified by non-equal crossing-over, replication slippage or via extrachromosomal circles [46], some of these mechanisms may differ between these two species. Unlike STAR-C, the distribution of TR1 and X.43.1 is conserved only in the genus Silene and Lychnis.

Distribution of repetitive sequences in genic regions compared to the whole genome – a lesson from microsatellites

From the previous studies it is known that microsatellites are strongly accumulated on S. latifolia sex chromosomes [22]. Surprisingly, based on genomic library screenings [28], microsatellites are underrepresented in the genomic repetitive DNA pool. This could either be due to cloning problems with tandem-arrayed DNA or misinterpretation of FISH data. Although the FISH method is a robust methodical approach in terms of generating rough estimations of DNA element localization, it does not usually provide information about small-scale patterns of distribution of individual elements. To focus on the distribution and constitution of microsatellites within sex chromosomes, we analyzed six BAC clones, each of which contained a sex linked gene and/or its autosomal homologue (Sl4, SlAP3) previously isolated both from S. latifolia and S. vulgaris [30], [31]. Our data suggest that there are no significant differences in microsatellite numbers either between sex chromosome-linked loci or between corresponding regions in S. latifolia and S. vulgaris (Table S2). Even when we focused on specific microsatellites, which were shown to be overrepresented on S. latifolia Y chromosome (CA, GA, GC, CAA, CAG, GAA, TAA) (Table S3), we did not find any significant differences in satellite distribution. Surprisingly, and in contrast to data by Kubat et al., some microsatellites (GA, GC, CAG, GAA, TAA) were more abundant in the X allele compared to the Y [22].

What happens if we compare the SlAP3Y locus to the Sl4Y locus in terms of microsatellite distribution? It has been estimated that the Sl4Y gene stopped recombining with its X linked counterpart about 7 Mya [34]. The SlAP3 gene is situated in a region in which recombination was restricted between 1–2.5 Mya [31]. Total counts of all microsatellites in both regions based on Table S2 reveal no differences in the general representation of microsatellites in these regions. This would suggest that microsatellites do not contribute to Y chromosome formation significantly, at least in some genic regions. On the other hand, our recent experiments focused on sequencing the S. latifolia genome have revealed frequent long reads (454 based sequencing) composed of only a repetition of a specific satellite motif. Data in this study suggest that microsatellite accumulation has either local character or covers mainly non-genic regions.

Materials and Methods

Plant material and DNA isolation

Plants of S. vulgaris and of S. latifolia were planted in a cultivation room under standard conditions (t 24°C, 16 h light/8 h dark). Genomic DNA was isolated from young leaves using DNAeasy Plant Mini Kit (Qiagen).

Construction and screening of genomic short insert plasmid and BAC libraries

S. vulgaris genomic DNA was processed by sonication into fragments with an average length of 600–1200 bp. Ends of fragments were treated with T4 DNA polymerase and further phosphorylated by T4 polynucleotide kinase. DNA fragments were ligated to the plasmid vector pSMART® LCAmp (Lucigen) using the Smart Cloning Kit (Lucigen) and transformed into E. cloni 10G competent cells. Clones were robotically picked with Genomic Solution G3 robot into 384 well plates, grown for 18 h, replicated, and frozen at −80°C. Clones were then grid in duplicate on Hybond N+ (Amersham, Biosciences) nitrocellulose membrane filters following a 4×4 pattern that allowed us to identify the well position and plate number of each clone. The filters were incubated and processed as described in [47]. The library contained 7,720 clones (603 bp average insert size) representing an equivalent of about 0.42% of the S. vulgaris genome. Screening was performed by radioactive hybridization with α32P and with the Prime-It II Random Primer Labelling Kit (Stratagene) according to the manufacturer's protocol. As a probe, the labeled S. vulgaris genomic DNA and amplified domains (with minor modifications according to [28]) Ogre, Retand and Copia reverse transcriptase, LINE endonuclease, and Au SINE. Mutator, Mariner, hAT and CACTA transposase gene and tandem repeats STAR-C, TR1, and X.43.1 were used. S. latifolia and S. vulgaris BAC libraries were screened according to Cegan et al. [31]. For BAC library screening probes derived from Sl4 and SlAP3 genes were used as described in Cegan et al. [31] and Marais et al. [30].

DNA amplification

Positively hybridized clones were selected and used as a template for PCR with vector specific SL1 and SR2 primers. The PCR reaction profile included 25 cycles of 30 s at 94°C, 30 s at 60°C and 1 min at 72°C preceded by an initial denaturation (3 min at 95°C) and followed by a final extension step (5 min at 72°C). For the repetitive DNA elements, PCR amplification was carried out according to [28]. For the Retand reverse transcriptase amplification we used POL primers under conditions described in [26], for the Ogre reverse transcriptase we used primers C233-F 5′-CCCTTTACCGCCACTGACTA-3′ and C233-R 5′-TCAGTTGGGTCTAGGGTCGT-3′. Cycling conditions for Ogre RT included an initial denaturation of 2 min at 94°C, 35 cycles of 40 s at 94°C, 40 s at 55°C, 40 s at 72°C and a final elongation of 7 min at 72°C. PTC-200 (MJ Research) and T3000 (Biometra, Goettingen, Germany) thermal cyclers were used.

Southern blot hybridization

Genomic DNA of S. latifolia (male and female) and of S. vulgaris were restricted by HindIII, MspI, and HpaII and then transferred by reverse Southern blotting onto Hybond N+ (Amersham, Biosciences) membrane filters. The Ogre, Retand and Copia S. latifolia reverse transcriptases were used as probes. Radioactive hybridization was performed as described in the construction and screening of the short insert library.

Sequencing and bioinformatics analysis

Amplified PCR products were treated by ExoSAP, labelled by BigDye® Terminator Cycle Sequencing Kit according to the manufacturer's instructions and further purified by Agencourt® CleanSEQ® kit. Purified and labeled samples were sequenced (Sanger sequencing) with a 96 capillary sequencer ABI 3730xl according to the manufacturer's instructions. Sequences are available under GenBank accession numbers JN624389-JN624685.

BAC DNA was isolated and commercially sequenced from selected BAC clones using 454 sequencing with Roche GS FLX (GATC Biotech, Konstanz). 454 reads were assembled using MIRA3 [48], TGICL [49] and Roche GS De novo Assembler version 2.5.3 software. BAC sequencing and assembly statistics are described in Table S4. BAC sequences are available under GenBank accession numbers JQ289120–JQ289125. BAC contig annotations, based on BLAST with xml output and conversion for Geneious Pro software (Biomatters Ltd, Auckland, New Zealand; [50]), were made using TAIR9 cds and TAIR9 TE databases, the S. vulgaris transcriptome database (Taylor et al., in preparation; http://silenegenomics.biology.virginia.edu/index.html). For graphic annotation in Geneious Pro sofware [50] we also used the vector and E. coli sequences, databases of short insert libraries of S. latifolia [28] and S. vulgaris (obtained in this paper), marker genes and gene prediction software Genscan [51].

Basic sequence analysis, sequence assembly and alignments were done with Geneious Pro software [50]. Multiple sequence comparisons were performed with MAFFT [52] (http://align.bmr.kyushu-u.ac.jp/mafft/online/server/) and BLAST online applications. A homology search was performed with BLAST [53]. Similarities with Repbase [54](http://www.giriinst.org/repbase/index.html) were found using CENSOR [55](http://www.giriinst.org/censor/index.php) and with Repeat Masker [56] (http://www.repeatmasker.org). For full length retroelement identification we used LTR Finder [57] (http://tlife.fudan.edu.cn/ltr_finder/) and JDotter [58] (http://athena.bioc.uvic.ca/QuickStart/JDotter). The other simple sequence analyses were completed using The Sequence Manipulation Suite - version 2 (SMS2) [59] (http://www.bioinformatics.org/sms2/). We used Bio-Linux 6.0 operating system [60].

Data analysis

Nucleotide alignment visualization of full length elements from BACs was done using Lalnview [61]. The comparative analysis of the Retand full length elements from BAC clones to genomic 454 reads from S. latifolia (male and female) was done on PROFREP server (beta version) (the server is maintained by the Laboratory of Molecular Cytogenetics, Institute of Plant Molecular Biology, Ceske Budejovice, Czech Republic) with E-value cutoff of 10−15.

To identify microsatellites, we used Perfect Microsatellite Repeat Finder (http://sgdp.iop.kcl.ac.uk/nikammar/repeatfinder.html) on each BAC sequence, with the default parameters (minimum number of repeats = 3, minimum repeat unit length = 2 and maximum repeat unit length = 100).

To calculate a number of sequences per genome, we took the actual number of each type of element in the library and multiplied it by genome coverage of the library. The % of the genome was calculated by multiplication of number of sequences per genome by average element size (kb) (Table 1, Table S1).

Fluorescence in situ hybridization on metaphase chromosomes

Slides with mitotic metaphase chromosomes of S. latifolia, S. vulgaris, S. dioica, Lychnis chalcedonica and Dianthus caryophyllus were treated as described in [62] with slight modifications. Slide denaturation was performed in 7∶3 (v/v) formamide: 2× SSC for 2 min at 72°C. Slides were immediately dehydrated through 50%, 70%, and 100% ethanol (−20°C), and air dried. The probe was denatured at 70°C for 10 min, and 100 ng of the denatured probe was added at room temperature and hybridized for 18 h at 37°C. Slides were analyzed using an Olympus Provis microscope, and image analysis was performed using ISIS software (Metasystems). DNA was labeled with Fluorolink Cy3-dUTP (Amersham Pharmacia Biotech) (red labeling) and Spectrum Green (Vysis) (green labeling) in combination with the nick translation mix (Roche).

The probe for STAR-C was synthesized by VBC-Genomics (Vienna) with Cy3 modification on the 5′end. Chromosomes were stained with DAPI (4′ 6′- diamidino-2-phenylindole).

Supporting Information

Figure S1

Southern blot analysis. Male (M) and female (F) genomic DNA of S. latifolia and S. vulgaris (SV) was restricted using HindIII, MspI and HpaII. Hybridization was carried out with reverse transcriptase of Ogre, Retand and Copia retroelements as probes. The 1 kb DNA ladder (L 1 kb) is indicated.

(TIF)

Click here for additional data file. (834.3KB, tif)
Figure S2

Chromosomal distribution of 25S rDNA (A–E) and tandem repeats STAR-C (F–J), TR1 (K–O) and X.43.1 (P–T) in five species from the Caryophyllaceae family as determined by FISH. Mitotic metaphase chromosomes of S. latifolia (A, F, K, P), S. dioica (B, G, L, Q), S. chalcedonica (C, H, M, R), S. vulgaris (D, I, N, S) and Dianthus caryophyllus (E, J, O, T) were counterstained with DAPI (blue). The probes were labeled with Cy3-conjugated nucleotides (red). The X and Y chromosomes are indicated, bars indicate 10 µm.

(TIF)

Click here for additional data file. (7MB, tif)
Figure S3

Comparison of STAR-C tandem repeat signal intensities in S. latifolia (A–C) and S. vulgaris (D–F) by FISH. Metaphase chromosomes were counterstained with DAPI (blue); the STAR-C probe was labeled with Cy3-conjugated nucleotides (red). Exposition time is indicated in the figure. The X and Y chromosomes are indicated, bar represents 10 µm.

(TIF)

Click here for additional data file. (1.9MB, tif)
Figure S4

Chromosomal distribution of STAR-C (red) and Retand (green) on L. chalcedonica. Mitotic metaphase chromosomes were counterstained with DAPI (blue).

(TIF)

Click here for additional data file. (1.7MB, tif)
Table S1

Proportions of individual elements in the genomes of S. latifolia and S. vulgaris (* data from Cermak et al. [28] ).

(PDF)

Click here for additional data file. (53.5KB, pdf)
Table S2

Percentage of microsatellites in genomic loci containing the SlAP3 and Sl4 genes in S. latifolia and S. vulgaris. Di-, tri-, and tetranucleotide microsatellites were calculated separately.

(PDF)

Click here for additional data file. (39.1KB, pdf)
Table S3

Percentage of specific microsatellites in BAC clones containing the SlAP3 and Sl4 genes in S. latifolia and S. vulgaris. Satellite units were selected based on data by Kubat et al. [20] showing accumulation of several microsatellites on Y chromosome of S. latifolia.

(PDF)

Click here for additional data file. (40.1KB, pdf)
Table S4

BAC sequencing and assembly statistics.

(PDF)

Click here for additional data file. (30.3KB, pdf)

Acknowledgments

We thank Dr. Julia M. Svoboda for critical reading of the manuscript.

Footnotes

Competing Interests: The authors have declared that no competing interests exist.

Funding: The work was supported by the Internal Grant Agency at Mendel University, Faculty of Agronomy, Brno, Czech Republic (grant no. DP7/2009), the Grant Agency of AS CR (KJB600040901), Czech Science Foundation (grant nos. P501/10/P483, P501/10/0102, 204/09/H002, P305/10/0930 and 522/09/0083) and the Academy of Sciences of the Czech Republic (grant nos. AV0Z50040507 and AV0Z50040702). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Associated Data

This section collects any data citations, data availability statements, or supplementary materials included in this article.

Supplementary Materials

Figure S1

Southern blot analysis. Male (M) and female (F) genomic DNA of S. latifolia and S. vulgaris (SV) was restricted using HindIII, MspI and HpaII. Hybridization was carried out with reverse transcriptase of Ogre, Retand and Copia retroelements as probes. The 1 kb DNA ladder (L 1 kb) is indicated.

(TIF)

Click here for additional data file. (834.3KB, tif)
Figure S2

Chromosomal distribution of 25S rDNA (A–E) and tandem repeats STAR-C (F–J), TR1 (K–O) and X.43.1 (P–T) in five species from the Caryophyllaceae family as determined by FISH. Mitotic metaphase chromosomes of S. latifolia (A, F, K, P), S. dioica (B, G, L, Q), S. chalcedonica (C, H, M, R), S. vulgaris (D, I, N, S) and Dianthus caryophyllus (E, J, O, T) were counterstained with DAPI (blue). The probes were labeled with Cy3-conjugated nucleotides (red). The X and Y chromosomes are indicated, bars indicate 10 µm.

(TIF)

Click here for additional data file. (7MB, tif)
Figure S3

Comparison of STAR-C tandem repeat signal intensities in S. latifolia (A–C) and S. vulgaris (D–F) by FISH. Metaphase chromosomes were counterstained with DAPI (blue); the STAR-C probe was labeled with Cy3-conjugated nucleotides (red). Exposition time is indicated in the figure. The X and Y chromosomes are indicated, bar represents 10 µm.

(TIF)

Click here for additional data file. (1.9MB, tif)
Figure S4

Chromosomal distribution of STAR-C (red) and Retand (green) on L. chalcedonica. Mitotic metaphase chromosomes were counterstained with DAPI (blue).

(TIF)

Click here for additional data file. (1.7MB, tif)
Table S1

Proportions of individual elements in the genomes of S. latifolia and S. vulgaris (* data from Cermak et al. [28] ).

(PDF)

Click here for additional data file. (53.5KB, pdf)
Table S2

Percentage of microsatellites in genomic loci containing the SlAP3 and Sl4 genes in S. latifolia and S. vulgaris. Di-, tri-, and tetranucleotide microsatellites were calculated separately.

(PDF)

Click here for additional data file. (39.1KB, pdf)
Table S3

Percentage of specific microsatellites in BAC clones containing the SlAP3 and Sl4 genes in S. latifolia and S. vulgaris. Satellite units were selected based on data by Kubat et al. [20] showing accumulation of several microsatellites on Y chromosome of S. latifolia.

(PDF)

Click here for additional data file. (40.1KB, pdf)
Table S4

BAC sequencing and assembly statistics.

(PDF)

Click here for additional data file. (30.3KB, pdf)

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