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. 1991 Nov 25;19(22):6133–6138. doi: 10.1093/nar/19.22.6133

Two independent promoters as well as 5' untranslated regions regulate Dd ras expression in Dictyostelium.

J F Louvion 1, J C Scholder 1, S Pinaud 1, C D Reymond 1
PMCID: PMC329103  PMID: 1659685

Abstract

The Dd ras gene produces three transcripts during Dictyostelium development. The largest transcript (L-) can be induced by external addition of cAMP even in cells prevented from aggregating, whereas shorter transcripts (S1- and S2-) expression requires cell aggregate formation. We show the presence of two independent promoters for L- and S-transcripts by deletion analysis of Dd ras fragments fused to CAT reporter genes reintroduced in Dictyostelium. A direct repeat upstream of S-transcript start sites which seems involved in S-promoter function, modulates also L-RNA accumulation. Furthermore removal of sequences between this repeat and the AUG protein start codon reduces the level of L-transcripts in aggregates. This study allowed to uncover the intricate pattern of sequences participating in the regulation of Dd ras expression.

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Selected References

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