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. 1991 Dec 11;19(23):6367–6372. doi: 10.1093/nar/19.23.6367

More than half of yeast U1 snRNA is dispensable for growth.

P G Siliciano 1, W J Kivens 1, C Guthrie 1
PMCID: PMC329179  PMID: 1754372

Abstract

Yeast U1 snRNA (568 nucleotides) is 3.5-fold larger than its mammalian counterpart (164 nucleotides) and contains apparent sequence homology only at the 5' and 3' ends. We have used deletion analysis to determine whether the yeast-specific U1 sequences play essential roles in vivo. Yeast cells carrying a deletion of more than 60% (355 nucleotides) of the single-copy U1 gene are viable, though slow-growing, while a deletion of 316 nucleotides allows essentially wild-type growth. The boundaries of the viable deletions define a dispensable internal domain which comprises sequences unique to yeast. In contrast, the essential 5' and 3' terminal domains correspond to phylogenetically conserved sequences and/or structures previously implicated in RNA:RNA and RNA:protein interactions. The minimal essential sequences of yeast U1 can be drawn in a secondary structure which resembles metazoan U1 in four of seven structural domains.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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