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. Author manuscript; available in PMC: 2013 Feb 28.
Published in final edited form as: Biochemistry. 2012 Feb 16;51(8):1617–1624. doi: 10.1021/bi3000935

Figure 4. The Gβ1γ1-dependent ADP-ribosylation of Gαt1′ and Gαt2′.

Figure 4

Pertussis toxin-catalyzed ADP-ribosylation of Gαt1′ and Gαt2′ (0.5 μM each) was carried out in the presence of increasing concentrations of Gβ1γ1. (A) [32P]ADP-ribosylation of Gαt1′ and Gαt2′ is analyzed by liquid scintillation counting (mean±SE, n=3). (B) Aliquots from the ADP-ribosylation reaction mixtures were analyzed by SDS-PAGE followed by autoradiography.