FIG. 5.

PKA RIIα overexpression attenuates the inhibitory effects of adiponectin on HSL Ser660 phosphorylation. 3T3-L1CARΔ1 cells were differentiated into adipocytes. Adenovirus vectors encoding GFP, PKA RIIα (A), or siRNA against PKA RIIα (B) were added to the medium. A: Cells were co-cultured 12 h later with Ad-Acrp30–transduced FAO cells, which secrete adiponectin. B: Some cells were treated with Bt2-cAMP for 1 h. Protein samples were prepared, and the phosphorylation levels of HSL Ser660 and other proteins were detected using specific antibodies. n = 6. Data are expressed as means ± SEM. *P < 0.05 vs. Ad-lacZ siRNA–treated cells at basal state.