Skip to main content
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1991 Dec 11;19(23):6653. doi: 10.1093/nar/19.23.6653

Heteroduplex formation as a means to exclude contamination in virus detection using PCR.

Y M Lo 1, E S Lo 1, P Patel 1, C H Tse 1, K A Fleming 1
PMCID: PMC329252  PMID: 1754411

Abstract

Full text

PDF

Page 6653

6653

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Clay T. M., Bidwell J. L., Howard M. R., Bradley B. A. PCR-fingerprinting for selection of HLA matched unrelated marrow donors. Collaborating Centres in the IMUST Study. Lancet. 1991 May 4;337(8749):1049–1052. doi: 10.1016/0140-6736(91)91704-x. [DOI] [PubMed] [Google Scholar]
  2. Gough N. M., Murray K. Expression of the hepatitis B virus surface, core and E antigen genes by stable rat and mouse cell lines. J Mol Biol. 1982 Nov 25;162(1):43–67. doi: 10.1016/0022-2836(82)90161-9. [DOI] [PubMed] [Google Scholar]
  3. Kaneko S., Feinstone S. M., Miller R. H. Rapid and sensitive method for the detection of serum hepatitis B virus DNA using the polymerase chain reaction technique. J Clin Microbiol. 1989 Sep;27(9):1930–1933. doi: 10.1128/jcm.27.9.1930-1933.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kaneko S., Miller R. H., Feinstone S. M., Unoura M., Kobayashi K., Hattori N., Purcell R. H. Detection of serum hepatitis B virus DNA in patients with chronic hepatitis using the polymerase chain reaction assay. Proc Natl Acad Sci U S A. 1989 Jan;86(1):312–316. doi: 10.1073/pnas.86.1.312. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kwok S., Higuchi R. Avoiding false positives with PCR. Nature. 1989 May 18;339(6221):237–238. doi: 10.1038/339237a0. [DOI] [PubMed] [Google Scholar]
  6. Lo Y. M., Mehal W. Z., Fleming K. A. In vitro amplification of hepatitis B virus sequences from liver tumour DNA and from paraffin wax embedded tissues using the polymerase chain reaction. J Clin Pathol. 1989 Aug;42(8):840–846. doi: 10.1136/jcp.42.8.840. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Nagamine C. M., Chan K., Lau Y. F. A PCR artifact: generation of heteroduplexes. Am J Hum Genet. 1989 Aug;45(2):337–339. [PMC free article] [PubMed] [Google Scholar]
  8. Saiki R. K., Gelfand D. H., Stoffel S., Scharf S. J., Higuchi R., Horn G. T., Mullis K. B., Erlich H. A. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 1988 Jan 29;239(4839):487–491. doi: 10.1126/science.2448875. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES