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. 1991 Dec 25;19(24):6691–6698. doi: 10.1093/nar/19.24.6691

Site-specific hydrolysis and alcoholysis of human immunodeficiency virus DNA termini mediated by the viral integrase protein.

C Vink 1, E Yeheskiely 1, G A van der Marel 1, J H van Boom 1, R H Plasterk 1
PMCID: PMC329296  PMID: 1662361

Abstract

Before integration of the human immunodeficiency virus (HIV) DNA, two nucleotides are removed from the 3' ends of the viral DNA by the integrase (IN) protein. We studied the chemistry of this reaction, and found that IN mediates site-specific hydrolysis of a phosphodiester bond, resulting in release of a dinucleotide. A class of alcohols (including glycerol, 1,2-propanediol, but not 1,3-propanediol) can also act as nucleophile in this reaction, and likewise the alcoholic amino acids L-serine and L-threonine can be covalently linked to the dinucleotide. No evidence was found for a covalent linkage between the IN protein and this dinucleotide, suggesting that IN directs a single nucleophilic attack of water at the specific phosphodiester bond.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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