Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1991 Dec 25;19(24):6713–6718. doi: 10.1093/nar/19.24.6713

P sequences of Drosophila subobscura lack exon 3 and may encode a 66 kd repressor-like protein.

N Paricio 1, M Pérez-Alonso 1, M J Martínez-Sebastián 1, R de Frutos 1
PMCID: PMC329299  PMID: 1662362

Abstract

Several P homologous sequences have been cloned and sequenced from Drosophila subobscura. These sequences are located at the 85DE region of the O chromosome and at least three of them are organized in tandem. We have identified four copies which exhibit strong similarity between them. All of the isolated elements are truncated at the 5' and 3' ends. They have lost the inverted terminal repeats and exon 3, but maintain exons 0, 1 and 2. They are transcribed producing a polyadenylated RNA. The structure of these transcripts suggests that they are able to encode a 66 kd repressor-like protein, but not a functional transposase. We ask about the biological role of a potential repressor protein in this species.

Full text

PDF
6713

Images in this article

6716Image
on p.6716

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anxolabéhère D., Kidwell M. G., Periquet G. Molecular characteristics of diverse populations are consistent with the hypothesis of a recent invasion of Drosophila melanogaster by mobile P elements. Mol Biol Evol. 1988 May;5(3):252–269. doi: 10.1093/oxfordjournals.molbev.a040491. [DOI] [PubMed] [Google Scholar]
  2. Anxolabéhère D., Nouaud D., Périquet G., Tchen P. P-element distribution in Eurasian populations of Drosophila melanogaster: A genetic and molecular analysis. Proc Natl Acad Sci U S A. 1985 Aug;82(16):5418–5422. doi: 10.1073/pnas.82.16.5418. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bingham P. M., Kidwell M. G., Rubin G. M. The molecular basis of P-M hybrid dysgenesis: the role of the P element, a P-strain-specific transposon family. Cell. 1982 Jul;29(3):995–1004. doi: 10.1016/0092-8674(82)90463-9. [DOI] [PubMed] [Google Scholar]
  4. Black D. M., Jackson M. S., Kidwell M. G., Dover G. A. KP elements repress P-induced hybrid dysgenesis in Drosophila melanogaster. EMBO J. 1987 Dec 20;6(13):4125–4135. doi: 10.1002/j.1460-2075.1987.tb02758.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Boussy I. A., Kidwell M. G. The P-M hybrid dysgenesis cline in Eastern Australian Drosophila melanogaster: discrete P, Q and M regions are nearly contiguous. Genetics. 1987 Apr;115(4):737–745. doi: 10.1093/genetics/115.4.737. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Brookfield J. F., Montgomery E., Langley C. H. Apparent absence of transposable elements related to the P elements of D. melanogaster in other species of Drosophila. 1984 Jul 26-Aug 1Nature. 310(5975):330–332. doi: 10.1038/310330a0. [DOI] [PubMed] [Google Scholar]
  7. Daniels S. B., Peterson K. R., Strausbaugh L. D., Kidwell M. G., Chovnick A. Evidence for horizontal transmission of the P transposable element between Drosophila species. Genetics. 1990 Feb;124(2):339–355. doi: 10.1093/genetics/124.2.339. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Daniels S. B., Strausbaugh L. D., Ehrman L., Armstrong R. Sequences homologous to P elements occur in Drosophila paulistorum. Proc Natl Acad Sci U S A. 1984 Nov;81(21):6794–6797. doi: 10.1073/pnas.81.21.6794. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Daniels S. B., Strausbaugh L. D. The distribution of P-element sequences in Drosophila: the willistoni and saltans species groups. J Mol Evol. 1986;23(2):138–148. doi: 10.1007/BF02099908. [DOI] [PubMed] [Google Scholar]
  10. Engels W. R. Germ line aberrations associated with a case of hybrid dysgenesis in Drosophila melanogaster males. Genet Res. 1979 Apr;33(2):137–146. doi: 10.1017/s0016672300018267. [DOI] [PubMed] [Google Scholar]
  11. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  12. Finnegan D. J., Fawcett D. H. Transposable elements in Drosophila melanogaster. Oxf Surv Eukaryot Genes. 1986;3:1–62. [PubMed] [Google Scholar]
  13. Hagemann S., Miller W. J., Pinsker W. P-related sequences in Drosophila bifasciata: a molecular clue to the understanding of P-element evolution in the genus Drosophila. J Mol Evol. 1990 Dec;31(6):478–484. doi: 10.1007/BF02102074. [DOI] [PubMed] [Google Scholar]
  14. Houck M. A., Clark J. B., Peterson K. R., Kidwell M. G. Possible horizontal transfer of Drosophila genes by the mite Proctolaelaps regalis. Science. 1991 Sep 6;253(5024):1125–1128. doi: 10.1126/science.1653453. [DOI] [PubMed] [Google Scholar]
  15. Karess R. E., Rubin G. M. Analysis of P transposable element functions in Drosophila. Cell. 1984 Aug;38(1):135–146. doi: 10.1016/0092-8674(84)90534-8. [DOI] [PubMed] [Google Scholar]
  16. Kaufman P. D., Doll R. F., Rio D. C. Drosophila P element transposase recognizes internal P element DNA sequences. Cell. 1989 Oct 20;59(2):359–371. doi: 10.1016/0092-8674(89)90297-3. [DOI] [PubMed] [Google Scholar]
  17. Kidwell M. G. Evolution of hybrid dysgenesis determinants in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1983 Mar;80(6):1655–1659. doi: 10.1073/pnas.80.6.1655. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kidwell M. G. Hybrid dysgenesis in Drosophila melanogaster: the genetics of cytotype determination in a neutral strain. Genetics. 1981 Jun;98(2):275–290. doi: 10.1093/genetics/98.2.275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Kidwell M. G., Kidwell J. F., Sved J. A. Hybrid Dysgenesis in DROSOPHILA MELANOGASTER: A Syndrome of Aberrant Traits Including Mutation, Sterility and Male Recombination. Genetics. 1977 Aug;86(4):813–833. doi: 10.1093/genetics/86.4.813. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Lansman R. A., Shade R. O., Grigliatti T. A., Brock H. W. Evolution of P transposable elements: sequences of Drosophila nebulosa P elements. Proc Natl Acad Sci U S A. 1987 Sep;84(18):6491–6495. doi: 10.1073/pnas.84.18.6491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Laski F. A., Rio D. C., Rubin G. M. Tissue specificity of Drosophila P element transposition is regulated at the level of mRNA splicing. Cell. 1986 Jan 17;44(1):7–19. doi: 10.1016/0092-8674(86)90480-0. [DOI] [PubMed] [Google Scholar]
  22. Misra S., Rio D. C. Cytotype control of Drosophila P element transposition: the 66 kd protein is a repressor of transposase activity. Cell. 1990 Jul 27;62(2):269–284. doi: 10.1016/0092-8674(90)90365-l. [DOI] [PubMed] [Google Scholar]
  23. Nitasaka E., Mukai T., Yamazaki T. Repressor of P elements in Drosophila melanogaster: Cytotype determination by a defective P element carrying only open reading frames 0 through 2. Proc Natl Acad Sci U S A. 1987 Nov;84(21):7605–7608. doi: 10.1073/pnas.84.21.7605. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. O'Hare K., Rubin G. M. Structures of P transposable elements and their sites of insertion and excision in the Drosophila melanogaster genome. Cell. 1983 Aug;34(1):25–35. doi: 10.1016/0092-8674(83)90133-2. [DOI] [PubMed] [Google Scholar]
  25. Rio D. C., Laski F. A., Rubin G. M. Identification and immunochemical analysis of biologically active Drosophila P element transposase. Cell. 1986 Jan 17;44(1):21–32. doi: 10.1016/0092-8674(86)90481-2. [DOI] [PubMed] [Google Scholar]
  26. Rio D. C. Molecular mechanisms regulating Drosophila P element transposition. Annu Rev Genet. 1990;24:543–578. doi: 10.1146/annurev.ge.24.120190.002551. [DOI] [PubMed] [Google Scholar]
  27. Rio D. C. Regulation of Drosophila P element transposition. Trends Genet. 1991 Sep;7(9):282–287. doi: 10.1016/0168-9525(91)90309-E. [DOI] [PubMed] [Google Scholar]
  28. Robertson H. M., Engels W. R. Modified P elements that mimic the P cytotype in Drosophila melanogaster. Genetics. 1989 Dec;123(4):815–824. doi: 10.1093/genetics/123.4.815. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Rubin G. M., Kidwell M. G., Bingham P. M. The molecular basis of P-M hybrid dysgenesis: the nature of induced mutations. Cell. 1982 Jul;29(3):987–994. doi: 10.1016/0092-8674(82)90462-7. [DOI] [PubMed] [Google Scholar]
  30. Rubin G. M., Spradling A. C. Genetic transformation of Drosophila with transposable element vectors. Science. 1982 Oct 22;218(4570):348–353. doi: 10.1126/science.6289436. [DOI] [PubMed] [Google Scholar]
  31. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Simonelig M., Anxolabéhère D. A P element of Scaptomyza pallida is active in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1991 Jul 15;88(14):6102–6106. doi: 10.1073/pnas.88.14.6102. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Stacey S. N., Lansman R. A., Brock H. W., Grigliatti T. A. Distribution and conservation of mobile elements in the genus Drosophila. Mol Biol Evol. 1986 Nov;3(6):522–534. doi: 10.1093/oxfordjournals.molbev.a040413. [DOI] [PubMed] [Google Scholar]
  34. Tabor S., Richardson C. C. DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. Proc Natl Acad Sci U S A. 1987 Jul;84(14):4767–4771. doi: 10.1073/pnas.84.14.4767. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES