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. Author manuscript; available in PMC: 2012 Mar 5.
Published in final edited form as: Differentiation. 2008 May 7;76(6):599–605. doi: 10.1111/j.1432-0436.2008.00275.x

Fig. 1.

Fig. 1

Isolation of UGSM-2 cells. The urogenital sinus of an E16 male INK4a −/− transgenic mouse embryo was subjected to collagenase digestion to separate epithelial (UGS-E) and mesenchymal (UGS-M) layers. UGS-M was placed into a culture dish and allowed to proliferate to confluence. Polyclonal UGSM cells were ring cloned to develop several stable clones, including the UGSM-2 cell line. Taken from Shaw et al. (2006).