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. 1991 Dec 25;19(24):6849–6854. doi: 10.1093/nar/19.24.6849

An efficient, sequence-specific method for crosslinking complementary oligonucleotides using binuclear platinum complexes.

E S Gruff 1, L E Orgel 1
PMCID: PMC329319  PMID: 1762915

Abstract

The binuclear PtII complexes [(trans-Pt(NH3)2Cl)2 (NH2(CH2)nNH2)]Cl2 (n = 4, 5 or 6), crosslink oligodeoxynucleotide-5'-phosphorothioates rapidly, specifically and efficiently to complementary single-stranded oligodeoxynucleotide targets. In the case that we investigated in detail, the most abundant crosslink is formed to the G residue complementary to the 5'-terminal C residue of the phosphorothioate. Less efficient crosslinking occurs to many other residues of the target. The same PtII complexes also bring about crosslinking efficiently to the polypurine tract in triple-helical DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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