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. 1991 May 11;19(9):2315–2320. doi: 10.1093/nar/19.9.2315

An RNA molecule copurifies with RNase P activity from Xenopus laevis oocytes.

M Doria 1, G Carrara 1, P Calandra 1, G P Tocchini-Valentini 1
PMCID: PMC329436  PMID: 1710353

Abstract

Utilizing a procedure for the purification of RNase P from Xenopus laevis germinal vesicle (GV) extracts, according to which the contamination by a large, cytoplasmic, cylindrical structure (1) is avoided, we demonstrate that the X.laevis enzyme, like the HeLa RNase P, is precipitated by anti-Th antibodies and an RNA molecule (XL RNA), 320 nucleotides long, copurifies with the activity. The sequence of XL RNA is 60% homologous to HeLa H1 RNA, therefore the two molecules seem related.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Akaboshi E., Guerrier-Takada C., Altman S. Veal heart ribonuclease P has an essential RNA component. Biochem Biophys Res Commun. 1980 Sep 30;96(2):831–837. doi: 10.1016/0006-291x(80)91430-8. [DOI] [PubMed] [Google Scholar]
  2. Arrigo A. P., Tanaka K., Goldberg A. L., Welch W. J. Identity of the 19S 'prosome' particle with the large multifunctional protease complex of mammalian cells (the proteasome). Nature. 1988 Jan 14;331(6152):192–194. doi: 10.1038/331192a0. [DOI] [PubMed] [Google Scholar]
  3. Bartkiewicz M., Gold H., Altman S. Identification and characterization of an RNA molecule that copurifies with RNase P activity from HeLa cells. Genes Dev. 1989 Apr;3(4):488–499. doi: 10.1101/gad.3.4.488. [DOI] [PubMed] [Google Scholar]
  4. Carrara G., Calandra P., Fruscoloni P., Doria M., Tocchini-Valentini G. P. Site selection by Xenopus laevis RNAase P. Cell. 1989 Jul 14;58(1):37–45. doi: 10.1016/0092-8674(89)90400-5. [DOI] [PubMed] [Google Scholar]
  5. Castaño J. G., Ornberg R., Koster J. G., Tobian J. A., Zasloff M. Eukaryotic pre-tRNA 5' processing nuclease: copurification with a complex cylindrical particle. Cell. 1986 Aug 1;46(3):377–385. doi: 10.1016/0092-8674(86)90658-6. [DOI] [PubMed] [Google Scholar]
  6. Chang D. D., Clayton D. A. A mammalian mitochondrial RNA processing activity contains nucleus-encoded RNA. Science. 1987 Mar 6;235(4793):1178–1184. doi: 10.1126/science.2434997. [DOI] [PubMed] [Google Scholar]
  7. Chang D. D., Clayton D. A. Mouse RNAase MRP RNA is encoded by a nuclear gene and contains a decamer sequence complementary to a conserved region of mitochondrial RNA substrate. Cell. 1989 Jan 13;56(1):131–139. doi: 10.1016/0092-8674(89)90991-4. [DOI] [PubMed] [Google Scholar]
  8. Doersen C. J., Guerrier-Takada C., Altman S., Attardi G. Characterization of an RNase P activity from HeLa cell mitochondria. Comparison with the cytosol RNase P activity. J Biol Chem. 1985 May 25;260(10):5942–5949. [PubMed] [Google Scholar]
  9. Donis-Keller H., Maxam A. M., Gilbert W. Mapping adenines, guanines, and pyrimidines in RNA. Nucleic Acids Res. 1977 Aug;4(8):2527–2538. doi: 10.1093/nar/4.8.2527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. England T. E., Bruce A. G., Uhlenbeck O. C. Specific labeling of 3' termini of RNA with T4 RNA ligase. Methods Enzymol. 1980;65(1):65–74. doi: 10.1016/s0076-6879(80)65011-3. [DOI] [PubMed] [Google Scholar]
  11. Fahnestock S. R., Nomura M. Activity of ribosomes containing 5S RNA with a chemically modified 3'-terminus. Proc Natl Acad Sci U S A. 1972 Feb;69(2):363–365. doi: 10.1073/pnas.69.2.363. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Falkenburg P. E., Haass C., Kloetzel P. M., Niedel B., Kopp F., Kuehn L., Dahlmann B. Drosophila small cytoplasmic 19S ribonucleoprotein is homologous to the rat multicatalytic proteinase. Nature. 1988 Jan 14;331(6152):190–192. doi: 10.1038/331190a0. [DOI] [PubMed] [Google Scholar]
  13. Forster A. C., Altman S. Similar cage-shaped structures for the RNA components of all ribonuclease P and ribonuclease MRP enzymes. Cell. 1990 Aug 10;62(3):407–409. doi: 10.1016/0092-8674(90)90003-w. [DOI] [PubMed] [Google Scholar]
  14. Gandini-Attardi D., Baldi I. M., Mattoccia E., Tocchini-Valentini G. P. Transfer RNA splicing endonuclease from Xenopus laevis. Methods Enzymol. 1990;181:510–517. doi: 10.1016/0076-6879(90)81148-n. [DOI] [PubMed] [Google Scholar]
  15. Gardiner K., Pace N. R. RNase P of Bacillus subtilis has a RNA component. J Biol Chem. 1980 Aug 25;255(16):7507–7509. [PubMed] [Google Scholar]
  16. Gold H. A., Craft J., Hardin J. A., Bartkiewicz M., Altman S. Antibodies in human serum that precipitate ribonuclease P. Proc Natl Acad Sci U S A. 1988 Aug;85(15):5483–5487. doi: 10.1073/pnas.85.15.5483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Gold H. A., Topper J. N., Clayton D. A., Craft J. The RNA processing enzyme RNase MRP is identical to the Th RNP and related to RNase P. Science. 1989 Sep 22;245(4924):1377–1380. doi: 10.1126/science.2476849. [DOI] [PubMed] [Google Scholar]
  18. Guerrier-Takada C., Gardiner K., Marsh T., Pace N., Altman S. The RNA moiety of ribonuclease P is the catalytic subunit of the enzyme. Cell. 1983 Dec;35(3 Pt 2):849–857. doi: 10.1016/0092-8674(83)90117-4. [DOI] [PubMed] [Google Scholar]
  19. Guerrier-Takada C., van Belkum A., Pleij C. W., Altman S. Novel reactions of RNAase P with a tRNA-like structure in turnip yellow mosaic virus RNA. Cell. 1988 Apr 22;53(2):267–272. doi: 10.1016/0092-8674(88)90388-1. [DOI] [PubMed] [Google Scholar]
  20. James B. D., Olsen G. J., Liu J. S., Pace N. R. The secondary structure of ribonuclease P RNA, the catalytic element of a ribonucleoprotein enzyme. Cell. 1988 Jan 15;52(1):19–26. doi: 10.1016/0092-8674(88)90527-2. [DOI] [PubMed] [Google Scholar]
  21. Johnson J. D., Ogden R., Johnson P., Abelson J., Dembeck P., Itakura K. Transcription and processing of a yeast tRNA gene containing a modified intervening sequence. Proc Natl Acad Sci U S A. 1980 May;77(5):2564–2568. doi: 10.1073/pnas.77.5.2564. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Kline L., Nishikawa S., Söll D. Partial purification of RNase P from Schizosaccharomyces pombe. J Biol Chem. 1981 May 25;256(10):5058–5063. [PubMed] [Google Scholar]
  23. Lerner M. R., Steitz J. A. Snurps and scyrps. Cell. 1981 Aug;25(2):298–300. doi: 10.1016/0092-8674(81)90047-7. [DOI] [PubMed] [Google Scholar]
  24. Martini G., Tato F., Gandini Attardi D., Tocchini-Valentini G. P. Nuclear localization of DNA polymerase alpha in Xenopus laevis oocytes. Biochem Biophys Res Commun. 1976 Oct 4;72(3):875–879. doi: 10.1016/s0006-291x(76)80213-6. [DOI] [PubMed] [Google Scholar]
  25. Miller D. L., Martin N. C. Characterization of the yeast mitochondrial locus necessary for tRNA biosynthesis: DNA sequence analysis and identification of a new transcript. Cell. 1983 Oct;34(3):911–917. doi: 10.1016/0092-8674(83)90548-2. [DOI] [PubMed] [Google Scholar]
  26. Otsuka A., de Paolis A., Tocchini-Valentini G. P. Ribonuclease "XlaI," an activity from Xenopus laevis oocytes that excises intervening sequences from yeast transfer ribonucleic acid precursors. Mol Cell Biol. 1981 Mar;1(3):269–280. doi: 10.1128/mcb.1.3.269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Pace N. R., Smith D. Ribonuclease P: function and variation. J Biol Chem. 1990 Mar 5;265(7):3587–3590. [PubMed] [Google Scholar]
  28. Parker K. A., Steitz J. A. Determination of RNA-protein and RNA-ribonucleoprotein interactions by nuclease probing. Methods Enzymol. 1989;180:454–468. doi: 10.1016/0076-6879(89)80117-x. [DOI] [PubMed] [Google Scholar]
  29. Stark B. C., Kole R., Bowman E. J., Altman S. Ribonuclease P: an enzyme with an essential RNA component. Proc Natl Acad Sci U S A. 1978 Aug;75(8):3717–3721. doi: 10.1073/pnas.75.8.3717. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Ulbrich N., Wool I. G. Identification by affinity chromatography of the eukaryotic ribosomal proteins that bind to 5 S ribosomal ribonucleic acid. J Biol Chem. 1978 Dec 25;253(24):9049–9052. [PubMed] [Google Scholar]
  31. Wang M. J., Davis N. W., Gegenheimer P. Novel mechanisms for maturation of chloroplast transfer RNA precursors. EMBO J. 1988 Jun;7(6):1567–1574. doi: 10.1002/j.1460-2075.1988.tb02981.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Zaug A. J., Kent J. R., Cech T. R. A labile phosphodiester bond at the ligation junction in a circular intervening sequence RNA. Science. 1984 May 11;224(4649):574–578. doi: 10.1126/science.6200938. [DOI] [PubMed] [Google Scholar]

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