Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1991 May 11;19(9):2321–2323. doi: 10.1093/nar/19.9.2321

BsiY I, a novel thermophilic restriction endonuclease that recognizes 5' CCNNNNNNNGG 3' and the discovery of a wrongly sequenced site in pACYC177.

Y K Mok 1, D R Clark 1, K M Kam 1, P C Shaw 1
PMCID: PMC329437  PMID: 2041772

Abstract

A new type II restriction endonuclease designated BsiY I has been purified from a thermophilic soil Bacillus stearothermophilus strain. This enzyme recognizes and cleaves the highly degenerate sequence 5' CCNNNNN!NNGG 3'. During the identification of the recognition sequence of BsiY I, we discovered that there should be five G nucleotides instead of four at position 1227-1230 of the plasmid pACYC177.

Full text

PDF
2321

Images in this article

2322Image
on p.2322
2322Image
on p.2322
2322Image
on p.2322

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brown N. L., Smith M. A general method for defining restriction enzyme cleavage and recognition sites. Methods Enzymol. 1980;65(1):391–404. doi: 10.1016/s0076-6879(80)65050-2. [DOI] [PubMed] [Google Scholar]
  2. Kessler C., Manta V. Specificity of restriction endonucleases and DNA modification methyltransferases a review (Edition 3). Gene. 1990 Aug 16;92(1-2):1–248. doi: 10.1016/0378-1119(90)90486-b. [DOI] [PubMed] [Google Scholar]
  3. Mok Y. K., Clark D. R., Kam K. M., Shaw P. C. Restriction endonuclease from thermophilic bacterial species I. Isolation and characterization of BsiEI. Nucleic Acids Res. 1990 Aug 25;18(16):4954–4954. doi: 10.1093/nar/18.16.4954. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Mok Y. K., Clark D. R., Kam K. M., Shaw P. C. Restriction endonuclease from thermophilic bacterial species. II. Isolation and characterization of BsiBI. Nucleic Acids Res. 1990 Nov 25;18(22):6740–6740. doi: 10.1093/nar/18.22.6740. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Roberts R. J. Restriction enzymes and their isoschizomers. Nucleic Acids Res. 1990 Apr 25;18 (Suppl):2331–2365. doi: 10.1093/nar/18.suppl.2331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Rose R. E. The nucleotide sequence of pACYC177. Nucleic Acids Res. 1988 Jan 11;16(1):356–356. doi: 10.1093/nar/16.1.356. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Rose R. E. The nucleotide sequence of pACYC184. Nucleic Acids Res. 1988 Jan 11;16(1):355–355. doi: 10.1093/nar/16.1.355. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Selzer G., Som T., Itoh T., Tomizawa J. The origin of replication of plasmid p15A and comparative studies on the nucleotide sequences around the origin of related plasmids. Cell. 1983 Jan;32(1):119–129. doi: 10.1016/0092-8674(83)90502-0. [DOI] [PubMed] [Google Scholar]
  9. Shaw P. C., Yung M. H., Zhu R. H., Ho W. K., Ng T. B., Yeung H. W. Cloning of trichosanthin cDNA and its expression in Escherichia coli. Gene. 1991 Jan 15;97(2):267–272. doi: 10.1016/0378-1119(91)90061-f. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES