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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Cancer. 2011 Dec 2;118(13):3433–3445. doi: 10.1002/cncr.26621

Figure 1. HO-1 expression is BCR-ABL1-dependent in vivo and is heightened in CML blast crisis specimens.

Figure 1

(A). HO-1 protein expression is increased in blast-crisis CML. A tissue microarray containing bone marrow specimens from 10 chronic-phase patients, 8 accelerated-phase patients, and 17 blastic-phase patients, were stained using a HO-1 specific antibody. Two representative patients who were followed from chronic phase to blastic phase are shown in A. (B) Graphical representation of the percentage of patients with a significant number (>20% positive) of HO-1-positive cells. (C) HO-1 mRNA is upregulated in an animal model for CML. HO-1 mRNA expression was examined by qualitative RT-PCR using HO-1-specific primers in GFP-sorted cells from a recipient mouse transplanted with MigR1-GFP-BCR-ABL1 transduced bone marrow from a donor mouse. Transplant efficiency was verified by BCR-ABL1 and β-actin amplification.