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. 1989 Oct;84(4):1320–1324. doi: 10.1172/JCI114300

Uptake of plasma fibrinogen into the alpha granules of human megakaryocytes and platelets.

P Harrison 1, B Wilbourn 1, N Debili 1, W Vainchenker 1, J Breton-Gorius 1, A S Lawrie 1, J M Masse 1, G F Savidge 1, E M Cramer 1
PMCID: PMC329793  PMID: 2677051

Abstract

The origin of platelet alpha-granule fibrinogen (Fg), whether from endogeneous synthesis or exogeneous derivation, remains unknown. Although Fg biosynthesis by megakaryocytes (MK) has been suggested, recent studies have demonstrated that certain alpha-granular proteins originate primarily from plasma. To study the origin of alpha-granule Fg, platelet-associated Fg was measured by ELISA and Western blotting, and localized by immunofluorescence and immunoelectron microscopy in a patient with symptomatic congenital afibrinogenemia before and after replacement therapy with cryoprecipitate. alpha-Granule Fg was detected in the majority of platelets as early as 24 h postinfusion, suggesting that direct platelet uptake was occurring. Platelet Fg reached a maximum value of 42.5% of normal values at 3 d postinfusion and was localized in the alpha-granules, while plasma levels followed a typical half-life profile. Significant alpha-granule Fg was still detectable at 13 d postinfusion, with plasma Fg virtually absent. Studies on cultured CFU-MKs from the patient also confirmed that MKs can incorporate exogeneous Fg into alpha-granules. These results indicate that platelet alpha-granule Fg can be derived from the circulating plasma pool and that Fg uptake can occur in both platelets and MKs.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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