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. 1993 Jan;91(1):110–114. doi: 10.1172/JCI116158

Glucocorticoids stimulate rabbit proximal convoluted tubule acidification.

M Baum 1, R Quigley 1
PMCID: PMC330003  PMID: 8423211

Abstract

Glucocorticoids have an important role in renal acidification; however, a direct effect of glucocorticoids on proximal convoluted tubule (PCT) acidification has not been directly demonstrated. In the present in vitro microperfusion study PCT from animals receiving dexamethasone (600 micrograms/kg twice daily for 2 d and 2 h before killing) had a significantly higher rate of bicarbonate absorption than did controls (92.0 +/- 13.3 vs 59.9 +/- 3.2 pmol/mm.min, P < 0.01). To examine if glucocorticoids had a direct epithelial action, dexamethasone was added to the bath of PCT perfused in vitro. After 3 h of incubation in paired experiments 10(-6) M and 10(-5) M dexamethasone resulted in an approximately 30% stimulation in the rate of bicarbonate absorption. 10(-7) M dexamethasone and 10(-6) M aldosterone had no effect on bicarbonate absorption. The stimulation of acidification by 10(-5) M dexamethasone was blocked by actinomycin D and cycloheximide. These data are consistent with a direct effect of glucocorticoids on PCT acidification, and this effect is dependent upon protein synthesis.

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Selected References

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  1. Aperia A., Haldosén L. A., Larsson L., Gustafsson J. A. Ontogeny of triamcinolone-acetonide binding sites in outer cortical tissue from rat kidneys. Am J Physiol. 1985 Dec;249(6 Pt 2):F891–F897. doi: 10.1152/ajprenal.1985.249.6.F891. [DOI] [PubMed] [Google Scholar]
  2. Aperia A., Larsson L. Induced development of proximal tubular NaKATPase, basolateral cell membranes and fluid reabsorption. Acta Physiol Scand. 1984 Jun;121(2):133–141. doi: 10.1111/j.1748-1716.1984.tb07439.x. [DOI] [PubMed] [Google Scholar]
  3. Baum M., Quigley R. Prenatal glucocorticoids stimulate neonatal juxtamedullary proximal convoluted tubule acidification. Am J Physiol. 1991 Nov;261(5 Pt 2):F746–F752. doi: 10.1152/ajprenal.1991.261.5.F746. [DOI] [PubMed] [Google Scholar]
  4. Bidet M., Merot J., Tauc M., Poujeol P. Na+-H+ exchanger in proximal cells isolated from kidney. II. Short-term regulation by glucocorticoids. Am J Physiol. 1987 Nov;253(5 Pt 2):F945–F951. doi: 10.1152/ajprenal.1987.253.5.F945. [DOI] [PubMed] [Google Scholar]
  5. Burg M., Grantham J., Abramow M., Orloff J. Preparation and study of fragments of single rabbit nephrons. Am J Physiol. 1966 Jun;210(6):1293–1298. doi: 10.1152/ajplegacy.1966.210.6.1293. [DOI] [PubMed] [Google Scholar]
  6. Dubrovsky A. H., Nair R. C., Byers M. K., Levine D. Z. Renal net acid excretion in the adrenalectomized rat. Kidney Int. 1981 Apr;19(4):516–528. doi: 10.1038/ki.1981.49. [DOI] [PubMed] [Google Scholar]
  7. Farman N., Oblin M. E., Lombes M., Delahaye F., Westphal H. M., Bonvalet J. P., Gasc J. M. Immunolocalization of gluco- and mineralocorticoid receptors in rabbit kidney. Am J Physiol. 1991 Feb;260(2 Pt 1):C226–C233. doi: 10.1152/ajpcell.1991.260.2.C226. [DOI] [PubMed] [Google Scholar]
  8. Farman N., Vandewalle A., Bonvalet J. P. Autoradiographic determination of dexamethasone binding sites along the rabbit nephron. Am J Physiol. 1983 Mar;244(3):F325–F334. doi: 10.1152/ajprenal.1983.244.3.F325. [DOI] [PubMed] [Google Scholar]
  9. Freiberg J. M., Kinsella J., Sacktor B. Glucocorticoids increase the Na+-H+ exchange and decrease the Na+ gradient-dependent phosphate-uptake systems in renal brush border membrane vesicles. Proc Natl Acad Sci U S A. 1982 Aug;79(16):4932–4936. doi: 10.1073/pnas.79.16.4932. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hulter H. N., Licht J. H., Bonner E. L., Jr, Glynn R. D., Sebastian A. Effects of glucocorticoid steroids on renal and systemic acid-base metabolism. Am J Physiol. 1980 Jul;239(1):F30–F43. doi: 10.1152/ajprenal.1980.239.1.F30. [DOI] [PubMed] [Google Scholar]
  11. Hulter H. N., Sigala J. F., Sebastian A. Effects of dexamethasone on renal and systemic acid-base metabolism. Kidney Int. 1981 Jul;20(1):43–49. doi: 10.1038/ki.1981.102. [DOI] [PubMed] [Google Scholar]
  12. Kinsella J. L., Freiberg J. M., Sacktor B. Glucocorticoid activation of Na+/H+ exchange in renal brush border vesicles: kinetic effects. Am J Physiol. 1985 Feb;248(2 Pt 2):F233–F239. doi: 10.1152/ajprenal.1985.248.2.F233. [DOI] [PubMed] [Google Scholar]
  13. Kinsella J., Cujdik T., Sacktor B. Na+-H+ exchange activity in renal brush border membrane vesicles in response to metabolic acidosis: The role of glucocorticoids. Proc Natl Acad Sci U S A. 1984 Jan;81(2):630–634. doi: 10.1073/pnas.81.2.630. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mishina T., Scholer D. W., Edelman I. S. Glucocorticoid receptors in rat kidney cortical tubules enriched in proximal and distal segments. Am J Physiol. 1981 Jan;240(1):F38–F45. doi: 10.1152/ajprenal.1981.240.1.F38. [DOI] [PubMed] [Google Scholar]
  15. Murer H., Hopfer U., Kinne R. Sodium/proton antiport in brush-border-membrane vesicles isolated from rat small intestine and kidney. Biochem J. 1976 Mar 15;154(3):597–604. [PMC free article] [PubMed] [Google Scholar]
  16. Noronha-Blob L., Sacktor B. Inhibition by glucocorticoids of phosphate transport in primary cultured renal cells. J Biol Chem. 1986 Feb 15;261(5):2164–2169. [PubMed] [Google Scholar]
  17. Poujeol P., Vandewalle A. Phosphate uptake by proximal cells isolated from rabbit kidney: role of dexamethasone. Am J Physiol. 1985 Jul;249(1 Pt 2):F74–F83. doi: 10.1152/ajprenal.1985.249.1.F74. [DOI] [PubMed] [Google Scholar]
  18. Quigley R., Baum M. Effects of growth hormone and insulin-like growth factor I on rabbit proximal convoluted tubule transport. J Clin Invest. 1991 Aug;88(2):368–374. doi: 10.1172/JCI115312. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. SARTORIUS O. W., CALHOON D., PITTS R. F. The capacity of the adrenalectomized rat to secrete hydrogen and ammonium ions. Endocrinology. 1952 Nov;51(5):444–450. doi: 10.1210/endo-51-5-444. [DOI] [PubMed] [Google Scholar]
  20. Sapir D. G., Pozefsky T., Knochel J. P., Walser M. The role of alanine and glutamine in steroid-induced nitrogen wasting in man. Clin Sci Mol Med. 1977 Sep;53(3):215–220. doi: 10.1042/cs0530215. [DOI] [PubMed] [Google Scholar]
  21. Schuster V. L., Kokko J. P., Jacobson H. R. Angiotensin II directly stimulates sodium transport in rabbit proximal convoluted tubules. J Clin Invest. 1984 Feb;73(2):507–515. doi: 10.1172/JCI111237. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Welbourne T. C., Phenix P., Thornley-Brown C., Welbourne C. J. Triamcinolone activation of renal ammonia production. Proc Soc Exp Biol Med. 1976 Dec;153(3):539–542. doi: 10.3181/00379727-153-39587. [DOI] [PubMed] [Google Scholar]
  23. Wilcox C. S., Cemerikic D. A., Giebisch G. Differential effects of acute mineralo- and glucocorticosteroid administration on renal acid elimination. Kidney Int. 1982 Apr;21(4):546–556. doi: 10.1038/ki.1982.61. [DOI] [PubMed] [Google Scholar]

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