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. 1990 Jan 25;18(2):291–298. doi: 10.1093/nar/18.2.291

Yeast regulatory protein LEU3: a structure-function analysis.

K M Zhou 1, Y L Bai 1, G B Kohlhaw 1
PMCID: PMC330266  PMID: 2183176

Abstract

Eleven mutations resulting in partially deleted or truncated LEU3 protein were generated by linker insertion or other modifications at restriction sites, deletion of restriction fragments, or oligonucleotide-directed mutagenesis. Functional studies of these mutants showed the following: (i) A specific DNA binding region is contained within the 173 N-terminal residues, but other regions of the protein are required for optimal binding. (ii) Activation of LEU2 expression depends on the C-terminal 113 residues of the LEU3 protein. (iii) Deletion of part or all of a central section of LEU3 eliminates the ability of the LEU3 protein to respond to the co-activator alpha-isopropylmalate, i.e. creates an unmodulated activator. (iv) Overproduction of unmodulated activator slows down cell growth. (v) Specific deletion of two short acidic regions, including one with net charge - 19, has only minor effects on activation and modulation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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