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. 1990 Jan 25;18(2):337–343. doi: 10.1093/nar/18.2.337

Requirement of protein co-factor for the DNA-binding function of the human ski proto-oncogene product.

T Nagase 1, G Mizuguchi 1, N Nomura 1, R Ishizaki 1, Y Ueno 1, S Ishii 1
PMCID: PMC330272  PMID: 2183181

Abstract

We identified the human c-ski gene product (c-Ski) as a protein with the apparent molecular weight of 100,000, p100c-ski, by using a c-Ski-specific polyclonal antibody. p100c-ski was a nuclear protein and p100c-ski in nuclear extracts of Molt4 cells bound to calf thymus DNA cellulose, but the bacterially synthesized c-Ski did not, suggesting that Ski was associated with another protein(s) and that the Ski complex had DNA-binding activity. This hypothesis was supported by the finding that the bacterially synthesized Ski bounds to DNA cellulose after being mixed with a nuclear extract of Molt4 cells. By use of a series of deletion mutants of Ski synthesized in an in vitro translation system, two portions in Ski were found to be necessary for the DNA binding of the Ski complex: the N-proximal portion containing a cystein/histidine-rich domain and the C-terminal portion including a region rich in basic amino acids.

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Selected References

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