Norovirus infections in children under 5 years of age hospitalized due to the acute viral gastroenteritis in northeastern Poland

Abstract

The primary aim of this study was to evaluate the frequency and seasonality of norovirus infection in hospitalized Polish children under 5 years of age, and a secondary aim was to compare the clinical severity of norovirus and rotavirus disease. The prospective surveillance study was carried out from July 2009 through June 2010. Stool samples from 242 children hospitalized due to acute viral gastroenteritis were tested for rotavirus group A and adenovirus with commercial immunochromatographic test and for norovirus with EIA assay. Single norovirus infection was found in 35/242 (14.5%) patients and in a further 5 (2.1%) children as co-infection with rotavirus. Overall, norovirus was detected in 16.5% of stool specimens. Norovirus infections tended to peak from October to November and again from February to March. In autumn months and in February, the proportion of norovirus gastroenteritis cases was equal or even surpassed those of rotavirus origin. Both norovirus and rotavirus infections most commonly affected children between 12 and 23 months of age. The low-grade or no fever was significantly more common in children infected with norovirus (94.3%) compared to rotavirus cases (52.9%). Overall, norovirus gastroenteritis was less severe than rotavirus disease with regard to 20-point severity scale (p < 0.05). Noroviruses have emerged as a relevant cause of acute gastroenteritis in Polish children. There is a great need for introducing routine norovirus testing of hospitalized children with gastroenteritis.

Introduction

Recently, norovirus gastroenteritis has been considered to be second to rotavirus gastroenteritis as the cause of children’s hospital admissions worldwide [1–4]. After implementation of rotavirus vaccines into national immunization programs in the years 2006 and 2007, the role of norovirus gastroenteritis is expected to increase [5]. With regard to the overview of recent literature, norovirus gastroenteritis accounts for 6–48% of all acute gastroenteritis cases with an overall median of 14% [6]. Noroviruses are small (27–35 nm in diameter), round-shaped, non-capsulated particles which are transmitted from person to person via the faecal-oral route, but droplet transmission after vomiting episodes can also occur. The small infective dose (<10 viral particles), long-time shedding, multiple modes of transmission, wide genetic diversity of norovirus strains, and resistance to the environmental factors make them highly contagious [1, 7, 8]. Although norovirus gastroenteritis is commonly regarded as a mild, short-lasting intestinal disorder which resolves by itself, severe dehydration and subsequent hospitalization are common complications in very young and very old persons, especially in those immunocompromised and with underlying diseases.

Due to the non-specific clinical manifestation, the diagnosis of sporadic norovirus infection can be made by detection of viral nucleic acid using reverse transcription-polymerase chain reaction (RT-PCR) assays or by detection of viral antigens in faeces using the enzyme linked immunosorbent assays (ELISA) or the immunochromatographic (ICG) tests [9–11]. Recently, RT-PCR has been accepted as the “gold standard” in norovirus diagnostics, whereas ELISA or ICG testing are suitable, relatively inexpensive and rapid methods to determine norovirus gastroenteritis on a routine basis in order to undertake the necessary transmission-related precautions. In Poland, the solid epidemiological data on sporadic and outbreak norovirus infections are lacking due to the fact that noroviruses are not routinely searched for in diagnostic laboratories and, to date, there is no reference research laboratory to determine the currently circulating strains.

The primary aim of this study was to determine the frequency and seasonality of sporadic norovirus infections in Polish children under 5 years of age hospitalized due to the community-acquired, acute viral gastroenteritis in the 2009/2010 epidemic season and also to compare the clinical severity of rotavirus and norovirus disease. The epidemic season 2009/2010 is the third one since chargeable rotavirus vaccines have been recommended to Polish infants. In 2009, the rotavirus vaccination coverage in northeastern Poland accounted for approximately 30% of children up to 3 years of age.

Material and methods

Setting

The study was carried out in the 20-bed Department of Paediatric Infectious Diseases at University Children’s Hospital in Bialystok, Poland. The department is located in northeastern Poland and provides inpatient care for children with acute gastroenteritis who reside in Bialystok city and Bialystok district. An estimated catchment population accounts for approximately 8,200 children aged <5 years, representing about 50% of all children aged <5 years in our region.

Material

All 373 children less than 5 years of age admitted to our department due to the community-acquired, acute viral gastroenteritis between July 2009 and June 2010 were invited to participate in the study project. The acute viral gastroenteritis was considered as abrupt onset of diarrhoea (three or more episodes of watery stool in a period of 24 h) and/or vomiting with or without fever. Inclusion criteria were: age less than 5 years and informed consent obtained from parents or legal guardians. Patients with diarrhoea of over 7 days’ duration, an antibiotic treatment before presentation, underlying diseases or confirmed bacterial diarrhoea were excluded. Overall, 242 children were enrolled in the study.

Methods

For each child, stool samples were collected within 36 hours of admission. Rotavirus group A and adenovirus were tested in fresh faecal specimens on a routine basis by means of commercial, ICG assay (VIKIA Rota-Adeno, Biomerieux). Then, the same stool samples for norovirus detection were frozen and stored in <20°C for future simultaneous testing with the IDEA™Norovirus (DakoCytomation; Oxoid, Ltd) according to the manufacturer’s instruction.

To assess severity of illness the clinical data (duration and frequency of diarrhoea and vomiting and the presence of fever and dehydration) were determined. The severity of gastrointestinal disease was estimated with the numerical scoring system of Ruuska and Vesikari [12]. Rotavirus immunization status was also recorded.

Statistical analysis was performed with Statistica StatSoft, version 9. The Mann–Whitney U-test was used to compare quantitative variables and chi-squared test for comparison of proportions. All tests were two-tailed and the difference was considered statistically significant when p < 0.05.

The study was approved by the Research Ethical Committee of the Medical University in Bialystok.

Results

During the one-year surveillance period a total of 242 children (114 males) with acute viral gastroenteritis were enrolled. As we estimated, they accounted for approximately 3% of the catchment population. Nine out of 242 (3.7%) children were vaccinated against rotavirus disease. Stool samples obtained from enrolled children were tested for enteric viruses. The causative agent of viral gastroenteritis was detected in 105/242 (43.4%) cases. Single norovirus infection was found in 35/242 (14.5%) patients, rotavirus in 51/242 (21.1%) and adenovirus in 14/242 (5.8%) patients. Dual viral-viral co-infection was confirmed in a further 5/242 (2.1%) cases. To assess the norovirus gastroenteritis burden in Polish children, relative prevalence of major viral agents norovirus, rotavirus group A and adenovirus was determined in positive-tested faecal specimens.

Rotavirus group A was the most prevalent single agent and was detected in 48.6% (51/105) of stool samples, including one stool sample coming from a vaccinated child. Norovirus was present in 33.3% (35/105) and adenovirus in 13.3% (14/105) of the specimens. Dual norovirus and rotavirus infection was detected in 3.8% (4/105) of cases and dual norovirus and adenovirus infection in one patient (1%). Overall, norovirus was detected in 16.5% (40/242) of the samples tested.

Although norovirus gastroenteritis cases were recorded throughout the year, they tended to peak in autumn months from September to November and again from January to March (Fig. 1). In autumn months and in February, the proportion of norovirus gastroenteritis cases was equal or even surpassed those of rotavirus origin. Among 35 children diagnosed with sporadic norovirus disease, 80% (28/35) were hospitalized from October through March. The median age of children infected with norovirus and rotavirus was similar and accounted for 17 months (range 1–57) and 18 months (range 4–57), respectively. The age-adjusted distribution of norovirus gastroenteritis cases compared to rotavirus gastroenteritis cases is presented in Fig. 2. The majority of norovirus infections was recorded in children up to 2 years of age (74%). Both norovirus and rotavirus infections most commonly affected children between 12 and 23 months of age.

Fig. 1.

Monthly distribution of norovirus (NV) and rotavirus (RV) cases (a), and proportions (b) in enrolled children. The black bars indicate norovirus cases; empty bars indicate rotavirus cases

Fig. 2.

Age-adjusted distribution of norovirus (NV; black bars) and rotavirus (RV; empty bars) infections in enrolled children

As we assessed, norovirus disease was similar to rotavirus disease with regard to frequency and duration of diarrhoea and vomiting (Table 1). The low-grade or no fever was significantly more common in children infected with norovirus (94.3%) compared to rotavirus cases (52.9%). Overall, norovirus gastroenteritis was a less severe disease than rotavirus disease with regard to the 20-point severity scale (p < 0.05).

Table 1.

Clinical manifestation of norovirus (NV) and rotavirus (RV) infections according to 20-point severity score [12]

Clinical symptoms	Score	NV, n = 35; (%)	RV, n  = 51; (%)	p value
Duration of diarrhea (days)
0	0	0	0	0.17
1–4	1	30 (85.7)	35 (68.6)
5	2	1 (2.9)	5 (9.8)
≥6	3	4 (11.4)	11 (21.6)
Diarrhea episodes/24 h
0	0	0	0	0.09
1	1	16 (45.7)	12 (23.5)
2	2	10 (28.6)	22 (43.1)
≥3	3	9 (25.7)	17 (33.4)
Duration of vomiting (days)
No vomiting	0	1 (2.8)	5 (9.8)	0.13
1	1	15 (42.9)	19 (37.3)
2	2	12 (34.3)	9 (17.6)
≥3	3	7 (20.0)	18 (35.3)
Vomiting episodes/24 h
No vomiting	0	1 (2.8)	5 (9.8)	0.15
1	1	6 (17.1)	4 (7.8)
2–4	2	15 (42.9)	30 (58.8)
≥5	3	13 (37.1)	12 (23.6)
Fever (°C)
<37.0	0	25 (71.4)	15 (29.4)	0.002
37.1–38.4	1	8 (22.9)	12 (23.5)
38.5–38.9	2	2 (5.7)	13 (25.5)
≥39.0	3	0	11 (21.6)
Dehydration
None	0	0	0	0.40
1–5%	2	35 (100)	50 (98.0)
≥6%	3	0	1 (2.0)
Severity scorea
Mild	0–7	0	1 (2.0)	0.01
Moderate	8–13	30 (85.7)	28 (54.9)
Severe	14–20	5 (14.3)	22 (43.1)

^aAll children were hospitalized and therefore received an extra 2 points

Figures in bold highlight the highest value

Discussion

This study is the third one coming from our facility which deals with norovirus infections in Polish children [13, 14]. After searching the recent polish literature adhering to this topic, we found only one study based on data coming from a routine surveillance system on foodborne and waterborne norovirus outbreaks in Poland [15]. Recently, many reports have established the importance of noroviruses as a cause of outbreaks [16–18]. Community-acquired, sporadic cases of norovirus gastroenteritis have been known to a much less extent since individual cases are poorly diagnosed and reported. In Poland like in many other countries, noroviruses are not covered by routine analysis of stool samples from patients with gastroenteritis, and therefore the problem of norovirus infections has been underappreciated. However, as we have documented in the present study, the incidence of single norovirus gastroenteritis in Polish children under 5 years of age is relatively high and accounts for 14.5% of all hospitalized acute gastroenteritis cases and for 33.3% of those of specified aetiology. The norovirus positivity rate in hospitalized Polish children increased by 3% compared to the results of our pilot study performed in 2005 [13]. It is likely that the observed increase in relative frequency of norovirus gastroenteritis cases is due to implementing a rotavirus vaccination in Polish infants since the 2006–2007 epidemic season. Patel et al. [4], reviewing the results of recent studies in which molecular methods were applied for detection of enteric viruses, showed that noroviruses accounted for approximately 12% (range 4.4–30.7%) of severe gastroenteritis cases among children <5 years of age worldwide. In turn, Koopman [6], searching the recent literature results for prevalence of norovirus in hospitalized children with acute gastroenteritis across the world, calculated the median value at the level of 14%. To our knowledge, the lowest prevalence of sporadic norovirus gastroenteritis in European children was recorded in Hungary [19] at 6.5%, whereas the highest were in Italy [20] at 39.2% and in Finland [5] at 29%. Observed discrepancies in frequency of norovirus disease need to be interpreted with caution. Many factors such as study design and duration, age of patients, epidemic season or methods applied may influence the final results. The IDEA enzyme immunoassay applied in this study is considered as highly specific for some noroviruses, but generally not sensitive enough to detect a wide range of noroviruses [9, 10]. Assuming that the sensitivity of the IDEA norovirus assay for detecting norovirus in samples collected in sporadic cases of gastroenteritis ranges from 44.6 to 75.9% [9], the real incidence of norovirus gastroenteritis in the present study is expected to be higher. Our results confirm the relevance of norovirus second to rotavirus as the cause of severe gastroenteritis in young Polish children. A limitation of the recent study is the lack of the possibility to determine the molecular characteristics of circulating norovirus strains.

In the present study, norovirus gastroenteritis cases were detected all year round, except July. We observed two peak waves, one in September–October and the second one from January to March. The same seasonality pattern was observed in our previous study [13]. Norovirus infections preceded the epidemic increase of rotavirus cases by two months. Similar regularity concerning winter predominance of norovirus infections was observed by many in previous studies [5, 20–23].

The overall severity score was significantly lower for norovirus infection compared to rotavirus gastroenteritis cases. This is probably due to a lower frequency of high-grade fever and evacuations in norovirus infected children. Similar results were found by Junquera et al. [3] in Spain and by Narkeviciute and Tamusauskaite [24] in Lithuania.

In conclusion, in light of recent findings, noroviruses have emerged as a relevant cause of acute, community-acquired gastroenteritis in Polish children. There is a great need for introducing the routine norovirus testing in hospitalized patients with gastroenteritis and to find at least one National Virus Reference Laboratory to recognize the real burden as well as molecular epidemiology of viral intestinal infections in the Polish childhood population.