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. 1990 Feb 25;18(4):785–792. doi: 10.1093/nar/18.4.785

SLACS retrotransposon from Trypanosoma brucei gambiense is similar to mammalian LINEs.

S Aksoy 1, S Williams 1, S Chang 1, F F Richards 1
PMCID: PMC330328  PMID: 2156231

Abstract

We have characterized a retrotransposon in Trypanosoma brucei gambiense uniquely associated with the spliced-leader (SL) RNA gene cluster (Spliced Leader Associated Conserved Sequence, SLACS). There are nine copies of SLACS and DNA sequence analysis of one shows the hallmarks of Line-1 like elements. SLACS has generated a 49 bp target DNA duplication at its insertion site and its 3'-end is preceded by a poly(A) stretch. Two putative open reading frames (ORFs) span 75% of the element. ORF1 has CysHis motif associated with the retroviral gag polypeptide while ORF2 shows homology with reverse transcriptase sequences. Its 5'-end contains a repeated segment of a 185 bp that varies in copy number in different SLACS insertions. Retrotransposon-like sequences inserted into the SL-RNA genes occur in several hemoflagellates. These elements may represent a related family which has maintained its target site specificity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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