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. 1990 Feb 25;18(4):1015–1020. doi: 10.1093/nar/18.4.1015

Immunoglobulin heavy chain gene organization and complexity in the skate, Raja erinacea.

F A Harding 1, N Cohen 1, G W Litman 1
PMCID: PMC330358  PMID: 2107524

Abstract

Immunoglobulin heavy chain genes from Raja erinacea have been isolated by cross hybridization with probes derived from the immunoglobulin genes of Heterodontus francisci (horned shark), a representative of a different elasmobranch order. Heavy chain variable (VH), diversity (DH) and joining (JH) segments are linked closely to constant region (CH) exons, as has been described in another elasmobranch. The nucleotide sequence homology of VH gene segments within Raja and between different elasmobranch species is high, suggesting that members of this phylogenetic subclass may share one VH family. The organization of immunoglobulin genes segments is diverse; both VD-J and VD-DJ joined genes have been detected in the genome of non-lymphoid cells. JH segment sequence diversity is high, in contrast to that seen in a related elasmobranch. These data suggest that the clustered V-D-J-C form of immunoglobulin heavy chain organization, including germline joined components, may occur in all subclasses of elasmobranchs. While variation in VH gene structure is limited, gene organization appears to be diverse.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blin N., Stafford D. W. A general method for isolation of high molecular weight DNA from eukaryotes. Nucleic Acids Res. 1976 Sep;3(9):2303–2308. doi: 10.1093/nar/3.9.2303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Davis M. M., Bjorkman P. J. T-cell antigen receptor genes and T-cell recognition. Nature. 1988 Aug 4;334(6181):395–402. doi: 10.1038/334395a0. [DOI] [PubMed] [Google Scholar]
  3. Feinberg A. P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. doi: 10.1016/0003-2697(83)90418-9. [DOI] [PubMed] [Google Scholar]
  4. Hinds K. R., Litman G. W. Major reorganization of immunoglobulin VH segmental elements during vertebrate evolution. Nature. 1986 Apr 10;320(6062):546–549. doi: 10.1038/320546a0. [DOI] [PubMed] [Google Scholar]
  5. Hood L., Kronenberg M., Hunkapiller T. T cell antigen receptors and the immunoglobulin supergene family. Cell. 1985 Feb;40(2):225–229. doi: 10.1016/0092-8674(85)90133-3. [DOI] [PubMed] [Google Scholar]
  6. Kobayashi K., Tomonaga S., Kajii T. A second class of immunoglobulin other than IgM present in the serum of a cartilaginous fish, the skate, Raja kenojei: isolation and characterization. Mol Immunol. 1984 May;21(5):397–404. doi: 10.1016/0161-5890(84)90037-3. [DOI] [PubMed] [Google Scholar]
  7. Kobayashi K., Tomonaga S., Teshima K., Kajii T. Ontogenic studies on the appearance of two classes of immunoglobulin-forming cells in the spleen of the Aleutian skate, Bathyraja aleutica, a cartilaginous fish. Eur J Immunol. 1985 Sep;15(9):952–956. doi: 10.1002/eji.1830150916. [DOI] [PubMed] [Google Scholar]
  8. Kobayashi K., Tomonaga S. The second immunoglobulin class is commonly present in cartilaginous fish belonging to the order Rajiformes. Mol Immunol. 1988 Feb;25(2):115–120. doi: 10.1016/0161-5890(88)90058-2. [DOI] [PubMed] [Google Scholar]
  9. Kokubu F., Hinds K., Litman R., Shamblott M. J., Litman G. W. Complete structure and organization of immunoglobulin heavy chain constant region genes in a phylogenetically primitive vertebrate. EMBO J. 1988 Jul;7(7):1979–1988. doi: 10.1002/j.1460-2075.1988.tb03036.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kokubu F., Hinds K., Litman R., Shamblott M. J., Litman G. W. Extensive families of constant region genes in a phylogenetically primitive vertebrate indicate an additional level of immunoglobulin complexity. Proc Natl Acad Sci U S A. 1987 Aug;84(16):5868–5872. doi: 10.1073/pnas.84.16.5868. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kokubu F., Litman R., Shamblott M. J., Hinds K., Litman G. W. Diverse organization of immunoglobulin VH gene loci in a primitive vertebrate. EMBO J. 1988 Nov;7(11):3413–3422. doi: 10.1002/j.1460-2075.1988.tb03215.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Litman G. W., Berger L., Murphy K., Litman R., Hinds K., Erickson B. W. Immunoglobulin VH gene structure and diversity in Heterodontus, a phylogenetically primitive shark. Proc Natl Acad Sci U S A. 1985 Apr;82(7):2082–2086. doi: 10.1073/pnas.82.7.2082. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Sakano H., Hüppi K., Heinrich G., Tonegawa S. Sequences at the somatic recombination sites of immunoglobulin light-chain genes. Nature. 1979 Jul 26;280(5720):288–294. doi: 10.1038/280288a0. [DOI] [PubMed] [Google Scholar]
  14. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Tonegawa S. Somatic generation of antibody diversity. Nature. 1983 Apr 14;302(5909):575–581. doi: 10.1038/302575a0. [DOI] [PubMed] [Google Scholar]
  16. Yancopoulos G. D., Alt F. W. Developmentally controlled and tissue-specific expression of unrearranged VH gene segments. Cell. 1985 Feb;40(2):271–281. doi: 10.1016/0092-8674(85)90141-2. [DOI] [PubMed] [Google Scholar]

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