Table 1. A single immunization with recombinant adenovirus encoding vaccinia virus glycoprotein A27L elicits complete protection against a virulent respiratory poxvirus infection.

A33R	Forward 5′- CACCGGATCCATGATGACACCAGAAAACGACGAAG -3′ (BamHI) Reverse 5′- GAATTCTTAGTTCATTGTTTTAACAC -3′ (EcoRI)
A34R	Forward 5′- CACCGGATCCATGAAATCGCTTAATAGACAAACTG -3′ (BamHI) Reverse 5′- GAATTCTCACTTGTAGAATTTTTTAACAC -3′ (EcoRI)
A36R	Forward 5′- CACCGGATCCATGATGCTGGTACCTCTTATCACG -3′ (BamHI) Reverse 5′- GAATTCTCACACCAATGATACGACCGATG -3′ (EcoRI)
B5R	Forward 5′- CACCAGATCTATGAAAACGATTTCCGTTGTTACG -3′ (BgIII) Reverse 5′- CTGCAGTTACGGTAGCAATTTATGGAAC -3′ (PstI)
A27L	Forward 5′- CACCATGGGATCCATGGACGGAACTC -3′ (BamHI) Reverse 5′- GAATTCTTACTCATATGGACGCCGTC -3′ (EcoRI)
L1R	Forward 5′- CACCATGGGATCCATGGGTGCCGCAC -3′ (BamHI) Reverse 5′- GAATTCTCAGTTTTGCATATCCGTGG -3′ (EcoRI)

Primer sequences for the vaccinia virus proteins used for PCR amplification with highlighted “CACC” sequences and underlined restriction enzyme sites