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. 1990 Apr 11;18(7):1687–1691. doi: 10.1093/nar/18.7.1687

DNA recombination during PCR.

A Meyerhans 1, J P Vartanian 1, S Wain-Hobson 1
PMCID: PMC330584  PMID: 2186361

Abstract

PCR co-amplification of two distinct HIV1 tat gene sequences lead to the formation of recombinant DNA molecules. The frequency of such recombinants, up to 5.4% of all amplified molecules, could be decreased 2.7 fold by a 6 fold increase in Taq DNA polymerase elongation time. Crossover sites mapped essentially to three discrete regions suggesting specific Taq DNA polymerase pause or termination sites. PCR mediated recombination may be a problem when studying heterogeneous genetic material such as RNA viruses, multigene families, or repetitive sequences. This phenomenon can be exploited to create chimeric molecules from related sequences.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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