Table 1.
Identification of Differentially Expressed Proteins of the MIA PaCa-2-2 Cells Treated with CP-320626 by MALDI-TOF/TOF MS
| Spot No.a | Accession Number | Protein name | Scoreb | Sequence Coverage (%) | m/z | Pep(Score)c | No. of Matched peptides | Caculated MW/pI | Turnover rate (%)d | ||
|---|---|---|---|---|---|---|---|---|---|---|---|
| PMF | MS/MS | PMF | MS/MS | ||||||||
| Up-regulated protein of the MIA PaCa-2-2 cells Treated with CP-320626 | |||||||||||
| 1 | P27797 | Calreticulin | 74 | 104 | 40 | 5 | 2856.1499 | (163)CKDDEFTHLYTLIVRPDNTYEVK(185) (104) | 8 | 48283/4.29 | 55 |
| 5 | Q01105 | Protein SET | 54 | 35 | 23 | 8 | 1063.6176 | (76)LRQPFFQK(83) (20) | 5 | 33469/4.23 | 67 |
| 2304.0354 | (133)SGYRIDFYFDENPYFENK(150) (15) | ||||||||||
| 6 | P47755 | F-actin-capping protein subunit alpha-2 | 52 | 49 | 22 | 9 | 1197.7813 | (22)LLLNNDNLLR(37) (16) | 4 | 33157/5.57 | 46 |
| 2101.1854 | (38)FIIHAPPGEFNEVFNDVR(47) (33) | ||||||||||
| 12 | P22626 | Heterogeneous nuclear ribonucleoproteins A2/B1 | 84 | 68 | 31 | 8 | 1377.6936 | (214)GGGGNFGPGPGSNFR(228) (43) | 8 | 37464/8.97 | 57 |
| 1798.9808 | (23)LFIGGLSFETTEESLR(38) (25) | ||||||||||
| 13 | P63244 | Guanine nucleotide-binding protein subunit beta-2-like 1 | 61 | 51 | 27 | 5 | 1982.0167 | (140)YTVQDESHSEWVSCVR(155) (51) | 5 | 35511/7.60 | 47 |
| 16 | Q9Y2P8 | RNA 3′-terminal phosphate cyclase-like protein | 72 | 26 | 21 | 3 | 1684.8055 | (175)KVLKPIQLTDPGK(187) (26) | 6 | 41273/9.36 | 48 |
| 17 | Q7L523 | Ras-related GTP-binding protein A | 79 | 100 | 31 | 11 | 1615.0127 | (153) SNYNFEKPFLWLAR (166) (31) | 7 | 24579/7.01 | 52 |
| 1741.0387 | (100) K.NVPNWHR (106) (97) | ||||||||||
| 18 | P15531 | Nucleoside diphosphate kinase A | 58 | 32 | 34 | 7 | 1344.7903 | (7)TFIAIKPDGVQR918) (32) | 5 | 17309/5.83 | 52 |
| 20 | P60660 | Myosin light polypeptide 6 | 71 | 30 | 55 | 10 | 1904.1181 | (64)VLDFEHFLPMLQTVAK(79) (30) | 10 | 17090/4.56 | 74 |
|
| |||||||||||
| Down-regulated proteins of the MIA PaCa-2-2 cells Treated with CP-320626 | |||||||||||
| 2 | P31943 | Heterogeneous nuclear ribonucleoprotein H | 106 | 306 | 34 | 18 | 1504.5409 | (17)GLPWSCSADEVQR(29) (46) | 11 | 49484/5.89 | 69 |
| 1684.5941 | (99)HTGPNSPDTANDGFVR(114) (35) | ||||||||||
| 1841.6673 | (151)HTGPNSPDTANDGFVR(167) (64) | ||||||||||
| 1996.7140 | (300)ATENDIYNFFSPLNPVR(316) (100) | ||||||||||
| 2141.7573 | (356)YVELFLNSTAGASGGAYEHR(375) (60) | ||||||||||
| 3 | P31943 | Heterogeneous nuclear ribonucleoprotein H | 134 | 243 | 41 | 18 | 1504.6935 | (17)GLPWSCSADEVQR(29) (33) | 12 | 49484/5.89 | 67 |
| 1684.7972 | (99)HTGPNSPDTANDGFVR(114) (36) | ||||||||||
| 1841.9464 | (151)HTGPNSPDTANDGFVR(167) (65) | ||||||||||
| 1997.0244 | (300)ATENDIYNFFSPLNPVR(316) (64) | ||||||||||
| 2142.0843 | (356)YVELFLNSTAGASGGAYEHR(375) (44) | ||||||||||
| 4 | Q86VC0 | 40S ribosomal protein SA | 127 | 159 | 49 | 30 | 1203.7121 | (90)FAAATGATPIAGR(102) (31) | 13 | 32947/4.79 | 38 |
| 1614.9305 | (167)GAHSVGLMWWMLAR(180) (36) | ||||||||||
| 1741.0202 | (64)AIVAIENPADVSVISSR(60) (104) | ||||||||||
| 2081.1482 | (103)FTPGTFTNQIQAAFREPR(120) (34) | ||||||||||
| 2996.6506 | (129)ADHQPLTEASYVNLPTIALCNTDSPLR(155) (65) | ||||||||||
| 7 | P04083 | Annexin A1 | 124 | 197 | 42 | 17 | 1262.6369 | (114)TPAQFDADELR(124) (31) | 11 | 38918/6.57 | 52 |
| 1543.9049 | (59)GVDEATIIDILTKR(72) (66) | ||||||||||
| 1702.9325 | (129)GLGTDEDTLIEILASR(144) (59) | ||||||||||
| 2067.9365 | (186)GDRSEDFGVNEDLADSDAR(204) (42) | ||||||||||
| 8 | Q6GMP2 | Alpha-enolase | 106 | 36 | 41 | 7 | 1691.9124 | (407)YNQLLRIEEELGSK(420) (11) | 13 | 47481/7.01 | 42 |
| 2176.0736 | (234)AGYTDKVVIGMDVAASEFFR(253) (36) | ||||||||||
| 9 | P04406 | Glyceraldehyde-3-phosphate dehydrogenase | 74 | 104 | 30 | 9 | 1763.8319 | (310)LISWYDNEFGYSNR(323) (83) | 6 | 36201/8.57 | 65 |
| 2041.1514 | (67)LVINGNPITIFQERDPSK(84) (22) | ||||||||||
| 10 | P04406 | Glyceraldehyde-3-phosphate dehydrogenase | 79 | 74 | 49 | 4 | 1763.7314 | (310)LISWYDNEFGYSNR(323) (74) | 10 | 36201/8.57 | 52 |
| 11 | P04406 | Glyceraldehyde-3-phosphate dehydrogenase | 59 | 122 | 32 | 11 | 1763.8625 | (310)LISWYDNEFGYSNR(323) (104) | 8 | 36201/8.57 | 57 |
| 2595.414 | (163)VIHDNFGIVEGLMTTVHAITATQK(186) (55) | ||||||||||
| 14 | P18669 | Phosphoglycerate mutase 1 | 100 | 52 | 46 | 14 | 1935.9489 | (47)DAGYEFDICFTSVQKR(62) (22) | 8 | 28900/6.67 | 45 |
| 2425.2106 | (142)YADLTEDQLPSCESLKDTIAR(162) (30) | ||||||||||
| 15 | P18669 | Phosphoglycerate mutase 1 | 156 | 50 | 61 | 4 | 1150.6759 | (181)VLIAAHGNSLR(191) (50) | 11 | 28900/6.67 | 49 |
| 19 | P16949 | Stathmin | 57 | 65 | 15 | 8 | 1388.9877 | (15)ASGQAFELILSPR(27) (65) | 3 | 17292/5.76 | 31 |
| 21 | P14174 | Macrophage migration inhibitory factor | 58 | 68 | 40 | 32 | 1287.7532 | (2)PMFIVNTNVPR(12) (60) | 5 | 12639/7.74 | 53 |
| 1303.7592 | (2)PMFIVNTNVPR(12) (38) | ||||||||||
| 2995.5631 | (90)ISPDRVYINYYDMNAANVGWNNSTFA(115) (8) | ||||||||||
| 22 | P07737 | Profilin-1 | 62 | 198 | 33 | 22 | 1915.1441 | (39)TFVNITPAEVGVLVGKDR(56) (144) | 6 | 15216/8.44 | 59 |
| 1625.8061 | (76)DSLLQDGEFSMDLR(89) (54) | ||||||||||
a
As labeled on the 2-D gel (see Fig. 2A).
b
Molecular weight search (MOWSE) scores obtained by the combined search (PMF and lift data) that were assigned to specific protein identity using the Mascot engine. The tolerance for parent ion and daughter ion is 100 ppm and 0.3 Da, respectively. Fixed modification parameter, carbamidomethylation (C); variable modification parameters, oxidation (at Met).
c
Peptides identified by Ultraflex MALDI-TOF/TOF in the “lift” mode and individual peptide score (lift data) in the parentheses. The superscript number represents the start and end amino acid.