FIG. 3.

Effect of interleukin-1 receptor antagonist (IL-1RA) on galanin-like peptide (GALP)-induced changes in food intake (A), body weight (B) and the change (Δ) in core body temperature (C and D) in rats. GALP (1.5 nmol in 1 μl) or vehicle (1 μl saline) was administered intracerebroventricularly (icv) in rats. Three separate icv injections of 200 μg (in 2 μl) of IL-1RA or vehicle (2 μl saline) were given at one hour before, at the time of, and one hour post injection of GALP (or vehicle). Food intake was measured at 1 and 24 h, and body weight at 24 h after injections. Body temperature was monitored continuously by remote radiotelemetry for 24 h but for clarity, data are shown only for 0-10 h. Analysis of the change in core body temperature over 0-10 h after injections is illustrated in D) as the area under the curve (AUC C/h). Data are mean ± SEM for n = 8-13 rats per group. *, P < 0.05, **, P < 0.01, ***, P < 0.001 vs vehicle-treated animals and ^##, P < 0.01, ^###, P < 0.001 vs IL-1RA-treated animals.