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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Surgery. 2011 Dec 9;151(4):594–605. doi: 10.1016/j.surg.2011.10.001

Figure 4.

Figure 4

HB-EGF activates Akt and inhibits NFκB DNA binding in EC via the PI3K/Akt pathway. EC were subjected to anoxia for 60 min followed by reoxygenation for 4 h or 12 h. EC were treated with LY294002 (20 μM) or AG1478 (500 nM) for 30 min prior to HB-EGF addition. A) PI3K-dependent activation of Akt in EC by HB-EGF. Cytosolic extracts were subjected to immunoblot analysis using phospho-specific Akt antibodies (p-Akt). Membranes were stripped and blotted with anti-total Akt (T-Akt) antibodies. Data are representative of three independent experiments. B) Effects of HB-EGF on NFκB DNA binding in EC. NFκB DNA binding activity in nuclear extracts of EC was assessed using a TransAMTM NFκB kit. The level of NFκB activation (p65 binding to its cognate DNA sequence) is expressed as absorbance at 450 nm. Results represent mean ± SEM of three separate experiments. *p< 0.05 Students t-test..