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. Author manuscript; available in PMC: 2012 Mar 21.
Published in final edited form as: Chembiochem. 2010 Jun 14;11(9):1266–1272. doi: 10.1002/cbic.201000073

Figure 3.

Figure 3

Transfection of MRC5-V2 cells with luciferase reporter using a series of cationic amphipathic peptides (A). The transfection efficiency of the peptide containing Dap (LADap) is not significantly different than that of the analogous peptide containing histidine (LAH) and is much greater than that of the two peptides containing either lysine (LAK) or ornithine (LAO). * indicates differences are significant (p < 0.05) with respect to LADap. Transfection is sensitive to inhibition with bafilomycin A1 (B) indicating that the gene delivery process is dependent on endosomal acidification and that the vector is sensitive to the change in pH in the endosome. PEI mediated transfection is reduced by around 99% and peptide mediated transfection by between 76 and 87%. * indicates treatment reduced transfection efficiency (p < 0.05). Transfections experiments were performed in serum free media.