Fig. 6.
Survivin overexpression confers modest protection against PEITC-mediated inhibition of PC-3 cell migration. A: Representative images from Transwell chamber assay depicting migration by PC-3 cells transiently transfected with empty vector (abbreviated as PC -3/EV) or vector encoding for Survivin (abbreviated as PC-3/Survivin) and treated for 24 hours with DMSO (control) or the indicated concentrations of PEITC. B: Quantitation of migrated PC-3 cells from experiment shown in panel A. Experiment was repeated twice in duplicate and combined results are shown as mean ±SD ( n=4). Significantly different (P< 0.05) compared with acorresponding DMSO-treated control and bbetween empty vector -transfected cells and Survivin overexpressing cells at each dose (0, 2.5, and 5 μM PEITC) by one-way ANOVA followed by Bonferroni’s multiple comparison test.