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. 1990 Jul 11;18(13):3771–3776. doi: 10.1093/nar/18.13.3771

Editing of the wheat coxIII transcript: evidence for twelve C to U and one U to C conversions and for sequence similarities around editing sites.

J M Gualberto 1, J H Weil 1, J M Grienenberger 1
PMCID: PMC331076  PMID: 1695731

Abstract

The complete cDNA sequence corresponding to the wheat coxIII gene transcript (coding for subunit 3 of cytochrome oxidase) has been determined by a method involving cDNA synthesis using specific oligonucleotides as primers followed by PCR amplification, cloning and sequencing of the amplification products. In 12 different clones, the same 13 nucleotide modifications have been found as compared to the genomic mitochondrial DNA sequence. Among these modifications, 12 are C----U conversions which change codons identities, thereby increasing the homology between the wheat COXIII protein and the corresponding protein of non-plant organisms. The 13th modification is a silent U----C conversion which seems to be an unfrequent editing eventin plant mitochondria. Homologies can be found between sequences surrounding editing sites in the coxIII transcript and in other wheat mitochondrial transcripts. The presence of such homology suggests that these sequences could base-pair with a common RNA molecule which might be involved in editing site recognition.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abraham J. M., Feagin J. E., Stuart K. Characterization of cytochrome c oxidase III transcripts that are edited only in the 3' region. Cell. 1988 Oct 21;55(2):267–272. doi: 10.1016/0092-8674(88)90049-9. [DOI] [PubMed] [Google Scholar]
  2. Benne R., Van den Burg J., Brakenhoff J. P., Sloof P., Van Boom J. H., Tromp M. C. Major transcript of the frameshifted coxII gene from trypanosome mitochondria contains four nucleotides that are not encoded in the DNA. Cell. 1986 Sep 12;46(6):819–826. doi: 10.1016/0092-8674(86)90063-2. [DOI] [PubMed] [Google Scholar]
  3. Blum B., Bakalara N., Simpson L. A model for RNA editing in kinetoplastid mitochondria: "guide" RNA molecules transcribed from maxicircle DNA provide the edited information. Cell. 1990 Jan 26;60(2):189–198. doi: 10.1016/0092-8674(90)90735-w. [DOI] [PubMed] [Google Scholar]
  4. Chang D. D., Clayton D. A. Mouse RNAase MRP RNA is encoded by a nuclear gene and contains a decamer sequence complementary to a conserved region of mitochondrial RNA substrate. Cell. 1989 Jan 13;56(1):131–139. doi: 10.1016/0092-8674(89)90991-4. [DOI] [PubMed] [Google Scholar]
  5. Covello P. S., Gray M. W. RNA editing in plant mitochondria. Nature. 1989 Oct 19;341(6243):662–666. doi: 10.1038/341662a0. [DOI] [PubMed] [Google Scholar]
  6. Grabau E. A., Gengenbach B. G. Cytochrome oxidase subunit III gene from soybean mitochondria. Plant Mol Biol. 1989 Nov;13(5):595–597. doi: 10.1007/BF00027319. [DOI] [PubMed] [Google Scholar]
  7. Gray M. W., Cedergren R., Abel Y., Sankoff D. On the evolutionary origin of the plant mitochondrion and its genome. Proc Natl Acad Sci U S A. 1989 Apr;86(7):2267–2271. doi: 10.1073/pnas.86.7.2267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gualberto J. M., Domon C., Weil J. H., Grienenberger J. M. Structure and transcription of the gene coding for subunit 3 of cytochrome oxidase in wheat mitochondria. Curr Genet. 1990 Jan;17(1):41–47. doi: 10.1007/BF00313247. [DOI] [PubMed] [Google Scholar]
  9. Gualberto J. M., Lamattina L., Bonnard G., Weil J. H., Grienenberger J. M. RNA editing in wheat mitochondria results in the conservation of protein sequences. Nature. 1989 Oct 19;341(6243):660–662. doi: 10.1038/341660a0. [DOI] [PubMed] [Google Scholar]
  10. Gualberto J. M., Wintz H., Weil J. H., Grienenberger J. M. The genes coding for subunit 3 of NADH dehydrogenase and for ribosomal protein S12 are present in the wheat and maize mitochondrial genomes and are co-transcribed. Mol Gen Genet. 1988 Dec;215(1):118–127. doi: 10.1007/BF00331312. [DOI] [PubMed] [Google Scholar]
  11. Hiesel R., Schobel W., Schuster W., Brennicke A. The cytochrome oxidase subunit I and subunit III genes in Oenothera mitochondria are transcribed from identical promoter sequences. EMBO J. 1987 Jan;6(1):29–34. doi: 10.1002/j.1460-2075.1987.tb04714.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hiesel R., Wissinger B., Schuster W., Brennicke A. RNA editing in plant mitochondria. Science. 1989 Dec 22;246(4937):1632–1634. doi: 10.1126/science.2480644. [DOI] [PubMed] [Google Scholar]
  13. Kimelman D., Kirschner M. W. An antisense mRNA directs the covalent modification of the transcript encoding fibroblast growth factor in Xenopus oocytes. Cell. 1989 Nov 17;59(4):687–696. doi: 10.1016/0092-8674(89)90015-9. [DOI] [PubMed] [Google Scholar]
  14. Macfarlane J. L., Wahleithner J. A., Wolstenholme D. R. A gene for cytochrome c oxidase subunit III (COXIII) in broad bean mitochondrial DNA: structural features and sequence evolution. Curr Genet. 1990 Jan;17(1):33–40. doi: 10.1007/BF00313246. [DOI] [PubMed] [Google Scholar]
  15. Maniatis T., Reed R. The role of small nuclear ribonucleoprotein particles in pre-mRNA splicing. Nature. 1987 Feb 19;325(6106):673–678. doi: 10.1038/325673a0. [DOI] [PubMed] [Google Scholar]
  16. McCarty D. M., Hehman G. L., Hauswirth W. W. Nucleotide sequence of the Zea mays mitochondrial cytochrome oxidase subunit III gene. Nucleic Acids Res. 1988 Oct 25;16(20):9873–9873. doi: 10.1093/nar/16.20.9873. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Powell L. M., Wallis S. C., Pease R. J., Edwards Y. H., Knott T. J., Scott J. A novel form of tissue-specific RNA processing produces apolipoprotein-B48 in intestine. Cell. 1987 Sep 11;50(6):831–840. doi: 10.1016/0092-8674(87)90510-1. [DOI] [PubMed] [Google Scholar]
  18. Saiki R. K., Gelfand D. H., Stoffel S., Scharf S. J., Higuchi R., Horn G. T., Mullis K. B., Erlich H. A. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science. 1988 Jan 29;239(4839):487–491. doi: 10.1126/science.2448875. [DOI] [PubMed] [Google Scholar]
  19. Schuster W., Hiesel R., Wissinger B., Brennicke A. RNA editing in the cytochrome b locus of the higher plant Oenothera berteriana includes a U-to-C transition. Mol Cell Biol. 1990 May;10(5):2428–2431. doi: 10.1128/mcb.10.5.2428. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Schuster W., Unseld M., Wissinger B., Brennicke A. Ribosomal protein S14 transcripts are edited in Oenothera mitochondria. Nucleic Acids Res. 1990 Jan 25;18(2):229–233. doi: 10.1093/nar/18.2.229. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Schuster W., Wissinger B., Unseld M., Brennicke A. Transcripts of the NADH-dehydrogenase subunit 3 gene are differentially edited in Oenothera mitochondria. EMBO J. 1990 Jan;9(1):263–269. doi: 10.1002/j.1460-2075.1990.tb08104.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Simpson L., Shaw J. RNA editing and the mitochondrial cryptogenes of kinetoplastid protozoa. Cell. 1989 May 5;57(3):355–366. doi: 10.1016/0092-8674(89)90911-2. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Thomas S. M., Lamb R. A., Paterson R. G. Two mRNAs that differ by two nontemplated nucleotides encode the amino coterminal proteins P and V of the paramyxovirus SV5. Cell. 1988 Sep 9;54(6):891–902. doi: 10.1016/S0092-8674(88)91285-8. [DOI] [PMC free article] [PubMed] [Google Scholar]

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