Volume 300, January 2011
Volume 69, January 2011
Anderson M, Suh JM, Kim EY, Dryer SE. Functional NMDA receptors with atypical properties are expressed in podocytes. Am J Physiol Cell Physiol 300: C22–C32, 2011. First published August 25, 2010; doi:10.1152/ajpcell.00268.2010 (http://ajpcell.physiology.org/content/300/1/C22).—In results, the same confocal image was inadvertently presented twice in Fig. 1A. The image on the right has now been replaced with the appropriate one, and the correct image of Fig. 1 is shown below. The authors apologize for this error. The changes made do not affect the conclusions presented in this study.
Fig. 1.
Responses to N-methyl-d-aspartate (NMDA) in differentiated cells of an immortalized mouse podocyte cell line. A: confocal immunofluorescence images showing expression of NR1 subunit of NMDA receptors in podocyte cell lines. B: reciprocal coimmunoprecipitation of NR1 subunits and the podocyte marker synaptopodin (Synpo). Input lane shows diluted sample of lysate used for preparation of immunoprecipitates. No signals of appropriate molecular weight were seen in immunoprecipitates prepared with IgG. C: whole cell recordings from 2 different podocytes. A micropipette filled with 100 μM NMDA was placed adjacent to the recorded cells and used to deliver NMDA in response to brief pressure pulses (horizontal bars above traces). Once the pipette was placed, responses to NMDA in a given cell were very consistent, as shown by response to 3 separate NMDA applications in each of the 2 cells. Vm, membrane (holding) potential. D: responses to NMDA do not desensitize or fade during prolonged application. Record shows a response to a continuous 60-s application of 100 μM NMDA to a podocyte at a holding potential of −60 mV. E: concentration-response curve for NMDA in podocytes. Data points show mean ± SE of currents evoked by a given NMDA concentration applied to groups of 4 cells. Superimposed curve is a nonlinear least-squares fit to the logistic equation with an EC50 of 36 μM NMDA and a Hill coefficient of 1.98 (R2 = 0.96413).