FIG 4 .

Effect of AnxA5 on LPS-induced production of TNF-α in vitro and in vivo. (A) In vitro, mouse macrophages treated with 1 ng/ml LPS and 0.2 µg/ml AnxA5 produced significantly less TNF-α than did cells treated with 1 ng/ml LPS alone and cells treated with a mixture of 1 ng/ml LPS and 0.04 ng/ml AnxA5. (B) In vivo, mice intravenously infused with LPS (1 µg/kg of body weight) with AnxA5 (100 µg/kg of body weight) had significantly lower serum TNF-α levels than mice infused with LPS alone; these levels were not significantly (N.S) different from those of mice infused with control normal saline or with AnxA5 alone.