Figure 2.

CHR-6494 treatment decreases cell viability, causes an arrest in G2/M and later apoptosis. (a) Effect of CHR-6494 in cell viability determined by the XTT assay in HCT-116, HeLa and MDA-MB-231 cells. IC₅₀ values are shown for each cell line. (b) Effect of CHR-6494 in the cell cycle. The percentage of G₂/M cells is CHR-6494 dose dependent, indicating that progression through mitosis is delayed. (c) The proapoptotic effect of CHR-6494. Upon Haspin inhibitor use, the percentage of cell death increases in the three cancer cell lines, as demonstrated by incorporation of Annexin V.