FIGURE 4.

Hnrpab disruption increases sensitivity of cells to glutamate-stimulated excitotoxicity. At 15DIV, Hnrpab^+/− and Hnrpab^−/− neurons were treated with 50 μM glutamate for 10 min, or mock treated with the same media changes lacking glutamate, and allowed to recover for 6 h. Cells were then fixed and imaged using 60× magnification, and cell death was scored visually through shrinkage of the nucleus in the DAPI channel and morphology of neurons using DIC microscopy. Error bars represent the standard deviation over three independent experiments. P-values were determined using a two-way ANOVA, followed by the Tukey test for multiple comparisons.