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. 1990 Aug 11;18(15):4417–4421. doi: 10.1093/nar/18.15.4417

High speed DNA sequencing by capillary electrophoresis.

J A Luckey 1, H Drossman 1, A J Kostichka 1, D A Mead 1, J D'Cunha 1, T B Norris 1, L M Smith 1
PMCID: PMC331259  PMID: 2388826

Abstract

A major challenge of the Human Genome Initiative is the development of a rapid, accurate, and efficient DNA sequencing technology. A major limitation of current technology is the relatively long time required to perform the gel electrophoretic separations of DNA fragments produced in the sequencing reactions. We demonstrate here that it is possible to increase the speed of sequence analysis by over an order of magnitude by performing the electrophoresis and detection in ultra thin capillary gels. An instrument which utilizes these high speed separations to simultaneously analyze many samples will constitute a second generation automated DNA sequencer suitable for large-scale sequence analysis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cheng Y. F., Dovichi N. J. Subattomole amino acid analysis by capillary zone electrophoresis and laser-induced fluorescence. Science. 1988 Oct 28;242(4878):562–564. doi: 10.1126/science.3140381. [DOI] [PubMed] [Google Scholar]
  2. Drossman H., Luckey J. A., Kostichka A. J., D'Cunha J., Smith L. M. High-speed separations of DNA sequencing reactions by capillary electrophoresis. Anal Chem. 1990 May 1;62(9):900–903. doi: 10.1021/ac00208a003. [DOI] [PubMed] [Google Scholar]
  3. Ewing A. G., Wallingford R. A., Olefirowicz T. M. Capillary electrophoresis. Anal Chem. 1989 Feb 15;61(4):292A–303A. doi: 10.1021/ac00179a002. [DOI] [PubMed] [Google Scholar]
  4. Gyllensten U. B. PCR and DNA sequencing. Biotechniques. 1989 Jul-Aug;7(7):700–708. [PubMed] [Google Scholar]
  5. Smith L. M., Fung S., Hunkapiller M. W., Hunkapiller T. J., Hood L. E. The synthesis of oligonucleotides containing an aliphatic amino group at the 5' terminus: synthesis of fluorescent DNA primers for use in DNA sequence analysis. Nucleic Acids Res. 1985 Apr 11;13(7):2399–2412. doi: 10.1093/nar/13.7.2399. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Smith L. M., Kaiser R. J., Sanders J. Z., Hood L. E. The synthesis and use of fluorescent oligonucleotides in DNA sequence analysis. Methods Enzymol. 1987;155:260–301. doi: 10.1016/0076-6879(87)55021-2. [DOI] [PubMed] [Google Scholar]
  7. Smith L. M., Sanders J. Z., Kaiser R. J., Hughes P., Dodd C., Connell C. R., Heiner C., Kent S. B., Hood L. E. Fluorescence detection in automated DNA sequence analysis. Nature. 1986 Jun 12;321(6071):674–679. doi: 10.1038/321674a0. [DOI] [PubMed] [Google Scholar]
  8. Wilson R. K., Chen C., Hood L. Optimization of asymmetric polymerase chain reaction for rapid fluorescent DNA sequencing. Biotechniques. 1990 Feb;8(2):184–189. [PubMed] [Google Scholar]

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