Plaque assay showing an increase in FHV titers in inoculated leaves of
MP-transgenic N. benthamiana plants. Leaves harvested 12
days after inoculation were homogenized and assayed for infectious FHV
as described in the text. Five microliters of 1-ml homogenates derived
from 100 mg of leaf tissues were used in each assay after appropriate
dilutions (100-fold for nontransgenic and 1,000-fold for transgenic).
Plates: 1, buffer-inoculated transgenic; 2, FHV RNA-inoculated
nontransgenic; 3, FHV RNA-inoculated TMV-MP transgenic; 4, FHV
RNA-inoculated RCNMV-MP transgenic leaf homogenates.