Skip to main content
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1993 Jul 25;21(15):3603–3604. doi: 10.1093/nar/21.15.3603

DISEC-TRISEC: di- and trinucleotide-sticky-end cloning of PCR-amplified DNA.

W Dietmaier 1, S Fabry 1, R Schmitt 1
PMCID: PMC331481  PMID: 8346048

Full text

PDF
3603

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aslanidis C., de Jong P. J. Ligation-independent cloning of PCR products (LIC-PCR). Nucleic Acids Res. 1990 Oct 25;18(20):6069–6074. doi: 10.1093/nar/18.20.6069. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Clark J. M. Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases. Nucleic Acids Res. 1988 Oct 25;16(20):9677–9686. doi: 10.1093/nar/16.20.9677. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Jones D. H., Sakamoto K., Vorce R. L., Howard B. H. DNA mutagenesis and recombination. Nature. 1990 Apr 19;344(6268):793–794. doi: 10.1038/344793a0. [DOI] [PubMed] [Google Scholar]
  4. Jung V., Pestka S. B., Pestka S. Efficient cloning of PCR generated DNA containing terminal restriction endonuclease recognition sites. Nucleic Acids Res. 1990 Oct 25;18(20):6156–6156. doi: 10.1093/nar/18.20.6156. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kaufman D. L., Evans G. A. Restriction endonuclease cleavage at the termini of PCR products. Biotechniques. 1990 Sep;9(3):304–306. [PubMed] [Google Scholar]
  6. Kovalic D., Kwak J. H., Weisblum B. General method for direct cloning of DNA fragments generated by the polymerase chain reaction. Nucleic Acids Res. 1991 Aug 25;19(16):4560–4560. doi: 10.1093/nar/19.16.4560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Mages W., Salbaum J. M., Harper J. F., Schmitt R. Organization and structure of Volvox alpha-tubulin genes. Mol Gen Genet. 1988 Aug;213(2-3):449–458. doi: 10.1007/BF00339615. [DOI] [PubMed] [Google Scholar]
  8. Marchuk D., Drumm M., Saulino A., Collins F. S. Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products. Nucleic Acids Res. 1991 Mar 11;19(5):1154–1154. doi: 10.1093/nar/19.5.1154. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Scharf S. J., Horn G. T., Erlich H. A. Direct cloning and sequence analysis of enzymatically amplified genomic sequences. Science. 1986 Sep 5;233(4768):1076–1078. doi: 10.1126/science.3461561. [DOI] [PubMed] [Google Scholar]
  10. Shuldiner A. R., Scott L. A., Roth J. PCR-induced (ligase-free) subcloning: a rapid reliable method to subclone polymerase chain reaction (PCR) products. Nucleic Acids Res. 1990 Apr 11;18(7):1920–1920. doi: 10.1093/nar/18.7.1920. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Vieira J., Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982 Oct;19(3):259–268. doi: 10.1016/0378-1119(82)90015-4. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES