Skip to main content
NCBI home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1989 Jan 11;17(1):31–36. doi: 10.1093/nar/17.1.31

The 4.5S RNA gene from Pseudomonas aeruginosa.

H Y Toschka 1, J C Struck 1, V A Erdmann 1
PMCID: PMC331533  PMID: 2492094

Abstract

The essential 4.5S RNA of Escherichia coli contains a structural motif, which is also present in RNAs from other organisms, i.e. Bacillus subtilis scRNA, Halobacterium halobium 7S RNA and eukaryotic 7SL RNAs. This suggests a common function in all organisms, which could be related to protein translocation, since 7SL RNA is essential for this process in eukaryotes. We have analysed the structure and expression of the 4.5S RNA gene from another gram-negative eubacterium, Pseudomonas aeruginosa. The single copy gene encodes a 113 nucleotides long RNA, which shares 75% sequence homology to the E. coli 4.5S RNA and also exhibits the completely conserved hairpin structure of the corresponding RNAs of B. subtilis and E. coli. Transcription initiates 24 nucleotides upstream from the mature 5' end and exceeds beyond the 4.5S RNA coding region. A distal open reading frame, similar to that described for E. coli, does not exist downstream from the P. aeruginosa 4.5S RNA gene.

Full text

PDF
31

Images in this article

34Image
on p.34

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bothwell A. L., Garber R. L., Altman S. Nucleotide sequence and in vitro processing of a precursor molecule to Escherichia coli 4.5 S RNA. J Biol Chem. 1976 Dec 10;251(23):7709–7716. [PubMed] [Google Scholar]
  2. Bourgaize D. B., Fournier M. J. Initiation of translation is impaired in E. coli cells deficient in 4.5S RNA. Nature. 1987 Jan 15;325(6101):281–284. doi: 10.1038/325281a0. [DOI] [PubMed] [Google Scholar]
  3. Brown S., Fournier M. J. The 4.5 S RNA gene of Escherichia coli is essential for cell growth. J Mol Biol. 1984 Sep 25;178(3):533–550. doi: 10.1016/0022-2836(84)90237-7. [DOI] [PubMed] [Google Scholar]
  4. Brown S. Mutations in the gene for EF-G reduce the requirement for 4.5S RNA in the growth of E. coli. Cell. 1987 Jun 19;49(6):825–833. doi: 10.1016/0092-8674(87)90620-9. [DOI] [PubMed] [Google Scholar]
  5. Grunstein M., Hogness D. S. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci U S A. 1975 Oct;72(10):3961–3965. doi: 10.1073/pnas.72.10.3961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hanahan D. Studies on transformation of Escherichia coli with plasmids. J Mol Biol. 1983 Jun 5;166(4):557–580. doi: 10.1016/s0022-2836(83)80284-8. [DOI] [PubMed] [Google Scholar]
  7. Harley C. B., Reynolds R. P. Analysis of E. coli promoter sequences. Nucleic Acids Res. 1987 Mar 11;15(5):2343–2361. doi: 10.1093/nar/15.5.2343. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hartmann R. K., Ulbrich N., Erdmann V. A. An unusual rRNA operon constellation: in Thermus thermophilus HB8 the 23S/5S rRNA operon is a separate entity from the 16S rRNA operon. Biochimie. 1987 Oct;69(10):1097–1104. doi: 10.1016/0300-9084(87)90009-5. [DOI] [PubMed] [Google Scholar]
  9. Hsu L. M., Zagorski J., Fournier M. J. Cloning and sequence analysis of the Escherichia coli 4.5 S RNA gene. J Mol Biol. 1984 Sep 25;178(3):509–531. doi: 10.1016/0022-2836(84)90236-5. [DOI] [PubMed] [Google Scholar]
  10. Ikemura T., Dahlberg J. E. Small ribonucleic acids of Escherichia coli. II. Noncoordinate accumulation during stringent control. J Biol Chem. 1973 Jul 25;248(14):5033–5041. [PubMed] [Google Scholar]
  11. Inouye M., Delihas N. Small RNAs in the prokaryotes: a growing list of diverse roles. Cell. 1988 Apr 8;53(1):5–7. doi: 10.1016/0092-8674(88)90480-1. [DOI] [PubMed] [Google Scholar]
  12. Melton D. A., Krieg P. A., Rebagliati M. R., Maniatis T., Zinn K., Green M. R. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res. 1984 Sep 25;12(18):7035–7056. doi: 10.1093/nar/12.18.7035. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Messing J., Gronenborn B., Müller-Hill B., Hans Hopschneider P. Filamentous coliphage M13 as a cloning vehicle: insertion of a HindII fragment of the lac regulatory region in M13 replicative form in vitro. Proc Natl Acad Sci U S A. 1977 Sep;74(9):3642–3646. doi: 10.1073/pnas.74.9.3642. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Platt T. Transcription termination and the regulation of gene expression. Annu Rev Biochem. 1986;55:339–372. doi: 10.1146/annurev.bi.55.070186.002011. [DOI] [PubMed] [Google Scholar]
  15. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Struck J. C., Toschka H. Y., Specht T., Erdmann V. A. Common structural features between eukaryotic 7SL RNAs, eubacterial 4.5S RNA and scRNA and archaebacterial 7S RNA. Nucleic Acids Res. 1988 Aug 11;16(15):7740–7740. doi: 10.1093/nar/16.15.7740. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Struck J. C., Vogel D. W., Ulbrich N., Erdmann V. A. The Bacillus subtilis scRNA is related to the 4.5S RNA from Escherichia coli. Nucleic Acids Res. 1988 Mar 25;16(6):2719–2719. doi: 10.1093/nar/16.6.2719. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Travers A. A. Conserved features of coordinately regulated E. coli promoters. Nucleic Acids Res. 1984 Mar 26;12(6):2605–2618. doi: 10.1093/nar/12.6.2605. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Walter P., Gilmore R., Blobel G. Protein translocation across the endoplasmic reticulum. Cell. 1984 Aug;38(1):5–8. doi: 10.1016/0092-8674(84)90520-8. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES