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. 1989 Jan 11;17(1):271–284. doi: 10.1093/nar/17.1.271

A 12 megabase restriction map at the cystic fibrosis locus.

T R Fulton 1, A M Bowcock 1, D R Smith 1, L Daneshvar 1, P Green 1, L L Cavalli-Sforza 1, H Donis-Keller 1
PMCID: PMC331550  PMID: 2911467

Abstract

We have constructed a physical map of the chromosomal region containing the cystic fibrosis locus using seven DNA markers and pulsed-field gel electrophoresis methods. The map includes cleavage sites for 8 rare-cutting restriction enzymes and spans over 12 megabases (Mb) of DNA, with one unlinked probe covering an additional 5 Mb. To our knowledge, this is the largest segment of human DNA which has been restriction-mapped to date. We can identify thirteen putative HTF islands spaced at intervals of 0.3-3.2 Mb. The region between loci D7S8 and MET, where the CF gene lies, includes 1.4-1.9 Mb of DNA.

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Selected References

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