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. 1989 Jan 11;17(1):335–354. doi: 10.1093/nar/17.1.335

Sequence requirements for branch formation in a group II self-splicing intron.

R Altura 1, B Rymond 1, B Seraphin 1, M Rosbash 1
PMCID: PMC331554  PMID: 2643082

Abstract

Evidence is presented for the existence of a specific intron-intron interaction, necessary for the formation of the branched product in the self-splicing reaction of a group II yeast mitochondrial intron. Trans-splicing reactions involving two RNA molecules (5' exon with covalently linked regions of intron and intron with covalently linked 3' exon) show that the presence of portions of intron domain I on the 5' molecule is necessary for the formation of branched products which are not seen with shorter 5' molecules. Modification/interference reactions show regions necesary for branch-formation and support a major role for specific regions of intron domain I. Further experiments, utilizing a truncated 3' molecule that is missing the conserved branchpoint nucleotide, indicate that domain VI may be required for a successful domain I interaction. A model for the formation of a proper branched structure includes implications for both cis and trans configurations.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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