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. 1989 Jan 25;17(2):617–629. doi: 10.1093/nar/17.2.617

Complex structural behavior of oligopurine-oligopyrimidine sequence cloned within the supercoiled plasmid.

P Parniewski 1, G Galazka 1, A Wilk 1, J Klysik 1
PMCID: PMC331607  PMID: 2644622

Abstract

Synthetic sequence GATCC(AG)7ATCG(AT)4CG(AG)7 was cloned into plasmid and its structural behavior under the influence of supercoiling was analysed by chemical modification at variety of experimental conditions. It was found that this sequence adopts at least two different non-B conformations depending on -delta and pH values. Moreover, 12 nucleotide long non-pur.pyr spacer region separating two identical (AG)7 blocks does not provide a significant energy barrier protecting against unusual structures formation.

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Selected References

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