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. 1989 Feb 25;17(4):1371–1377. doi: 10.1093/nar/17.4.1371

A catalytic 13-mer ribozyme.

A C Jeffries 1, R H Symons 1
PMCID: PMC331809  PMID: 2646593

Abstract

A 13-mer oligoribonucleotide can act as a ribozyme for the specific self-cleavage of a 41-mer oligoribonucleotide substrate in the presence of Mg2+. The two sequences involved correspond to the self-cleavage hammerhead structure of the virusoid of lucerne transient streak virus. The Michaelis-menten kinetic parameters for the reaction were; Km 1.3 microM, Vmax 0.012 microM min-1, kcat 0.5 min-1. The 13-mer RNA is the smallest ribozyme so far reported. A DNA analogue of the 13-mer can not substitute for the RNA in the reaction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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