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. 1989 Feb 25;17(4):1493–1509. doi: 10.1093/nar/17.4.1493

Action of a mammalian AP-endonuclease on DNAs of defined sequences.

B I Haukanes 1, D E Helland 1, K Kleppe 1
PMCID: PMC331817  PMID: 2466239

Abstract

An apurinic/apyrimidinic (AP) specific endonuclease from mouse plasmacytoma cells (line MPC-11), was observed to cleave apurinic sites in oligonucleotides 9, 11, 12, 39 and 40 nucleotides in length. However, the enzyme failed to cleave AP-sites in two oligonucleotides 7 nucleotides in length. The maximum rates of digestion observed on these short single-stranded DNA (ssDNA) fragments were approximately 1/30 of the rates observed on double-stranded DNA (dsDNA). In studies using the Maxam-Gilbert DNA sequencing analysis, apurinic sites in purine-rich regions were preferentially cleaved in dsDNA but not in ssDNA, indicating that the enzyme has a sequence preference on dsDNA. These results suggest that some sites on DNA might be more efficiently repaired than others.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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