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. 1994 Oct 11;22(20):4039–4043. doi: 10.1093/nar/22.20.4039

5-Nitroindole as an universal base analogue.

D Loakes 1, D M Brown 1
PMCID: PMC331887  PMID: 7937128

Abstract

4-, 5- and 6-Nitroindole have been investigated and compared with 3-nitropyrrole as universal bases in oligodeoxynucleotides. Of these the 5-nitroindole derivative was found to be superior giving higher duplex stability, and behaving indiscriminately towards each of the four natural bases in duplex melting experiments. 3-Nitropyrrole, whilst not discriminating between the natural bases, was found to lead to considerable destabilisation of the duplexes, particularly when multiple substitutions were made, in contrast to the 5-nitroindole nucleoside.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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