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. 1990 Aug 25;18(16):4825–4831. doi: 10.1093/nar/18.16.4825

Structural features of the priming signal recognized by primase: mutational analysis of the phage G4 origin of complementary DNA strand synthesis.

H Hiasa 1, H Sakai 1, T Komano 1, G N Godson 1
PMCID: PMC331955  PMID: 1697677

Abstract

45 mutations (insertion, deletion and base substitution) of the G4 Goric were tested for their functional activity in M13 and R199 in vivo. The critical mutants were also assayed for their ability to synthesize pRNA in vitro using SSB and primase. The results demonstrate that the secondary structure and spacing of stem-loops I and III are essential for Goric activity and that the 5'-CTG-3' sequence flanking stem-loop I is essential for initiation of pRNA synthesis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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